中国兽医科学2017，Vol.47Issue(10)：1245-1249,5.DOI:10.16656/j.issn.1673-4696.2017.10.007
猪流行性腹泻病毒间接ELISA检测方法的建立
Development and application of an indirect ELISA for detecting antibodies aginst porcine epidemic diarrhea virus
摘要
Abstract
The enzyme linked immunosorbent assay (ELISA) is a rapid diagnostic method,and is convenient for clinic practice.In this study,the N protein prokaryotic expression of PEDV AH2012 strain was established previously.And recombination N protein has been expressed.Furthermore,indirect ELISA method was established for the detection of IgG antibody,and each steps of method were optimized.The result showed this indirect ELISA was a stable,accurate method,and rarely influenced by environment factors,such as temperature.Test results indicated this ELISA method was sensitive and specific,and there were no cross-reactions with PRRSV,PRV,TGEV,FMDV,PCV2,PRCV positive sera.The intra-and inter-assay coefficients of variation were lower than 5.55％.The coincidence rate of results of the ELISA and the neutralization test was 88.75％.The ELISA method could be applied to the diagnosis of PEDV,PEDV epidemiological surveillance and vaccine antibodies detection.This ELISA method is a promising candidate for the detection and prevention of PEDV.关键词
猪流行性腹泻/N蛋白/抗体检测/间接ELISAKey words
porcine epidemic diarrhea/N protein/antibody detection/indirect ELISA分类
农业科技引用本文复制引用
孙冰,孙杰,徐向伟,俞正玉,郭容利,袁万哲,何孔旺,李彬..猪流行性腹泻病毒间接ELISA检测方法的建立[J].中国兽医科学,2017,47(10):1245-1249,5.基金项目
江苏省农业科技自主创新基金项目[CX(15)1056] （15）
