中国兽医科学2017，Vol.47Issue(10)：1264-1268,5.DOI:10.16656/j.issn.1673-4696.2017.10.010
新城疫病毒液相芯片技术检测方法的建立
Development of the detection method of Newcastle disease virus utilizing liquid chip technology
摘要
Abstract
To develop a detection method of Newcastle disease virus utilizing double-antibody sandwich liquid chip technology,the capture antibodies were conjugated to the 67 microshpere.The coupling capacity of capture antibodies,the working concentration of detection antibody and SA-PE were optimized.At the same time the specificity,sensitivity,stability and clinical samples of the method were analyzed.The optimal coupling capacity of capture antibody was 25 μ g of 1 × 106microspheres,and the optimal working concentration of detection antibody and SA-PE was 2 μg/mL and 4 μg/mL,respectively.The results showed that the method had not cross reactions with other viruses.The detection limit reached 0.0625HA,and the sensitivity was higher than HA.Reproducibility analysis showed that the in tra-and inter-assay coefficient of variation was 2.0％ and 6.7％,respectively.The detection rate of 60 clinical samples was 5％.Compared to PCR,there had a same detection rate.These results indicated that the method was high specificity,sensitivity and stability.It is a new method for detectiong Newcastle disease virus.关键词
新城疫病毒/液相芯片/检测方法Key words
Newcastle disease virus/liquid chip/detection method分类
农业科技引用本文复制引用
朱余军,陈梅丽,丛锋,饶丹,伍妙梨,肖丽,练月晓,黄碧洪,黄韧,郭鹏举..新城疫病毒液相芯片技术检测方法的建立[J].中国兽医科学,2017,47(10):1264-1268,5.基金项目
广东省科技计划项目(2015B070701003、2016A040403065) （2015B070701003、2016A040403065）
