中国兽医科学2017，Vol.47Issue(10)：1287-1291,5.DOI:10.16656/j.issn.1673-4696.2017.10.014
猪肠道病毒甲型3C基因的克隆和原核蛋白的表达
Cloning and prokaryotic expression of porcine enteriovirus A 3C gene
摘要
Abstract
Porcine enteriovirus A(PEV A)is a capsule-free,single-stranded positive strand RNA virus,belonging to the small RNA virus family,and can cause porcine polio,intestinal,respiratory,genital diseases and other multiple system syndromes.In this study,the full-length splicing primers were designed based on PAS (PCR-based accurate synthesis).The protective bases were designed at both ends of the primers.PEV A was synthesised and inserted into the expression vector pCzn1.The recombinant plasmid pCzn1-PEV A 3C was transferred into ArcticExpress expression strain.The target protein PEV A-3C was induced and obtained by Ni affinity chromatography.The results showed that the recombinant protein was successfully expressed and the molecular weight with the fusion protein was about 21.7 ku,which laid the foundation for the establishment of PEV A specific ELISA method.关键词
猪肠道病毒甲型/3C基因/蛋白表达Key words
porcine enteriovirus A,PEV A/3C gene/prokaryotic expression分类
农业科技引用本文复制引用
孙杰,李彬,朱琳,焦点,俞正玉,范宝超,袁万哲,王建辉,郭容利,何孔旺..猪肠道病毒甲型3C基因的克隆和原核蛋白的表达[J].中国兽医科学,2017,47(10):1287-1291,5.基金项目
江苏省农业科技自主创新资金项目[CX(15)1056] （15）
