中国兽医科学2017，Vol.47Issue(10)：1299-1302,4.DOI:10.16656/j.issn.1673-4696.2017.10.016
华支睾吸虫抗原CsSQ1和CsSQ2的免疫学虫体定位
Immunolocalization of antigens CsSQ1 and CsSQ2 of Clonorchis sinensis
摘要
Abstract
To study the localization and characteristics of the recombinant antigens of Clonorchis sinensis,two amino acids highly repetitive sequences were obtained by construction and screening of a cDNAexpression libraries of C.sinensis etacercaria and adult worms.Then we synthesized peptides with highly repetitive gene sequences and coupling with the carrier protein.Animals were immunized and sera was collected.Then antibodies were purified by ammonium sulfate precipitation and affinity chromatography.At last antigenic genes were located by immunofluorescence technique.In results,recombinant antigens CsSQ1 and CsSQ2 of C.sinensis mainly distributed in oral sucker and ventral suckers,respectively.The localization of the recombinant antigens of C.sinensis was confirmed.We speculate that the recombinant antigens may be related to the immune adhesion of C.sinensis.It provided the reference for the applied research in diagnosis and immunological characteristics of antigens of C.sinensis in the future.关键词
华支睾吸虫/重组抗原/特性/免疫学定位Key words
Clonorchis sinensis/recombinant antigen/characteristic/immunolocalization分类
农业科技引用本文复制引用
孙青松,于妮娜,尚信池,钱瑞程,李国江,刘明远..华支睾吸虫抗原CsSQ1和CsSQ2的免疫学虫体定位[J].中国兽医科学,2017,47(10):1299-1302,4.基金项目
吉林市科技发展计划资助项目(20166034) （20166034）
吉林农业科技学院博士启动基金项目(吉农院合字2015第243号) （吉农院合字2015第243号）
吉林农业科技学院种子基金项目(吉农院合字2016第Z01号) （吉农院合字2016第Z01号）
吉林农业科技学院校级重点学科培育项目(吉农合字2015第X055号) （吉农合字2015第X055号）
