中国兽医科学2017，Vol.47Issue(10)：1303-1308,6.DOI:10.16656/j.issn.1673-4696.2017.10.017
不同繁殖周期成年牦牛睾丸细胞外基质相关蛋白的分布比较
Distribution of extracellular matrix components in the adult yak testis during seasonal breeding cycle
摘要
Abstract
This study was conducted to characterize the histochemical characteristics and distribution of extracellular matrix proteins:laminin(LN),typeⅣcollagen(ColⅣ) and heparan sulfate proteoglycans(HSPG) in adult yak testis during different breeding seasons by Masson trichromatic dyeing,Gomori silver impregnation and immunohistochemical SP technique combined with Image-Pro Plus (IPP) statistical methods.The observations of light microscope found that the interstitial tissues were abundant with collagen and reticular fiber both in the breeding season and non-breeding season adult yak testis.Immunostaining analysis appeared that the LNwas present,being similar in Sertoli cells and spermatogenic cells in both different seasons,and the relative expression of LNwas almost the same between two different seasons although the expression in leydig cells during breeding season was reduced obviously(P＞0.05).The immunoreactivity for Col Ⅳwere seen in Sertolicells and spermatogenic cells in both seasons but the Col Ⅳdistribution near the lumen of seminiferous tubule in breeding yak showed to be strong positive,and the relative expression of Col Ⅳ in the breeding season yak testis tissue was more higher than that in non-breeding seasons(P＜0.01).In the adult yak testis,the HSPG expression was mainly strong in Sertoli cells and spermatogonium,and the average absorbance of HSPG expression was decreased significantly in non-breeding season(P＜0.01).Taken together,the changes of testis connective tissues in different breeding seasons are not obvious in yaks which live in plateau environment.The synthesis of LN changes with the secretion of leydig cells of different breeding season.In breeding season,the higher distribution of Col Ⅳare closely related to a bettercondition of spermatogenesis and the expression of HSPG is significant in the sertoli cell and spermatogonia differentiation.关键词
牦牛/睾丸/层黏连蛋白/胶原蛋白/硫酸乙酰肝素糖蛋白Key words
yak/testis/collagenⅣ/laminin/heparan sulfate proteoglycans分类
农业科技引用本文复制引用
袁莉刚,陈国娟,李聪,路琪中..不同繁殖周期成年牦牛睾丸细胞外基质相关蛋白的分布比较[J].中国兽医科学,2017,47(10):1303-1308,6.基金项目
国家自然科学基金项目(31160488) （31160488）
甘肃农业大学青年导师基金项目(GAU-QDNS-201604) （GAU-QDNS-201604）
