中国畜牧兽医2017，Vol.44Issue(9)：2558-2565,8.DOI:10.16431/j.cnki.1671-7236.2017.09.003
藏羊白细胞介素-7基因的PCR扩增及生物信息学分析
PCR Amplification and Bioinformatics Analysis of IL-7 Gene in Tibetan Sheep
摘要
Abstract
In order to get the interleukin-7 (IL-7) gene sequence of Tibetan sheep,and research the characteristics of this sequence and structure and function of encoding protein,IL-7 gene was amplified from Tibetan sheep by RT-PCR.The nucleotide sequence,amino acid sequence,homology and phylogenetic tree were analyzed by the DNAStar software.The secondary and tertiary structures,hydrophilicity,signal peptide and post-translational modification site of the encoding protein were predicted by DNAStar software and online servers.The results showed that the length of IL-7 gene was 531 bp (contained termination codon),and encoded 176 amino acids.Compared with IL-7 gene of Ovis aries,Capra hircus,Pantholops hodgsonii,Bubalus bubalis,Bos indicus,Bison bison bison,Bos taurus and Bos mutus,IL-7 gene of Tibetan sheep showed a great similarity from 97.2％ to 99.8％,the amino acid sequence homology varied from 94.9％ to 99.4％,and the relationship was the closest between Tibetan sheep and Ovis aries.Result from protein structure prediction indicated that the IL-7 protein was mainly composed of α-helix,it was a hydrophilic and secretory protein.Furthermore,it had six kinds of post translational modification sites,including one N-myristoylation site,one amidation site,one cAMP-and cGMP-de-pendent protein kinase phosphorylation site,three N-glycosylation sites,four protein kinase C phosphorylation sites and six casein kinase Ⅱ phosphorylation sites.These results might provide references for further study and clinical application of IL-7 gene in Tibetan sheep.关键词
藏羊/IL7基因/RT-PCR/序列分析/蛋白结构预测Key words
Tibetan sheep/IL-7 gene/RT-PCR/sequence analysis/protein structure prediction分类
生物科学引用本文复制引用
张晓芬,冶贵生..藏羊白细胞介素-7基因的PCR扩增及生物信息学分析[J].中国畜牧兽医,2017,44(9):2558-2565,8.基金项目
青海省科技厅项目(2016-ZJ-738) （2016-ZJ-738）
青海省“高端创新人才千人计划” （）
