中国畜牧兽医2017，Vol.44Issue(9)：2682-2691,10.DOI:10.16431/j.cnki.1671-7236.2017.09.021
应用iTRAQ技术对滩羊裘皮差异表达蛋白的研究
Study on Differentially Expressed Proteins of Tan Sheep Skin Using iTRAQ Technology
摘要
Abstract
In order to study the differential protein associated with the thickness and curvature of the flax fiber and the specific spike composition of Tan sheep,this study used the isotope labeling relative and absolute quantification (iTRAQ) proteomics methods,and combined with multidimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) identification and screening of skin protein from Tan sheep,Inner Mongolia Sunitou sheep,Zhongwei goat and Xinji Fine-wool sheep,and the Proteome Discoverer 1.4 software was used for quantitative analysis,combined with database search,identification of differential protein.Finally,the differential proteins was analyzed by the GO and Pathway analysis methods.1 161 proteins were identified from the four kinds of sheep skin,30 different proteins were found between Inner Mongolia Sunitou sheep and Tan sheep,112 differential proteins were found between Zhongwei goats and Tan sheep,107 differential proteins were found between Xinji Fine-wool sheep and Tan sheep,the skin differential proteins were analyzed for each group,it was found that the 14-3-3 protein sigma,KRT25,KRT34,KRT85,TCHH,KAP6 and KAP11.1 might be related to the thickness and curvature of the flax fiber and the formation of specific spike shape of Tan sheep.The result would provide a scientific basis for the breeding of Tan sheep fur quality.关键词
滩羊/皮肤/iTRAQ/差异表达蛋白Key words
Tan sheep/skin/iTRAQ/differentially expressed proteins分类
农业科技引用本文复制引用
杨佐青,陶金忠,李颖康,丁伟,刘永斌,魏炳栋..应用iTRAQ技术对滩羊裘皮差异表达蛋白的研究[J].中国畜牧兽医,2017,44(9):2682-2691,10.基金项目
宁夏农业育种专项“滩羊品系选育”项目(2013NYYZ0402) （2013NYYZ0402）
