中国畜牧兽医2017，Vol.44Issue(9)：2739-2746,8.DOI:10.16431/j.cnki.1671-7236.2017.09.028
合成绵羊抗菌肽NK-Lysin抗菌活性及其对雏鸡沙门氏菌攻毒的治疗效果研究
Antimicrobial Activity of Synthesized Sheep NK-Lysin Peptides and Its Treatment for Chicken Salmonella Challenge
摘要
Abstract
In order to study the biological function of the main active region of sheep antimicrobial peptide NK-Lysin,three pairs of functional domain peptide fragments were designed and synthesized the antibacterial activity was detected by radial diffusion test and minimal inhibitory concentration.We analyzed the toxic effects of chicken red blood cells and screen the best peptides for treatment chicken challenged by Salmonella pullorum in this study.The rusults showed that synthesized peptides were inhibitory to Escherichia coli and Salmonella pullorum.Fragment length of peptides,C-terminal amidation and peptide inner loop were essential to antibacterial activity.Two of synthesized peptides were used to treat chichen challenged by Salmonella pullorum which obviously decreased the mortality of chicken.The pathological damage of heart,liver,kidney were less than that of control group.This study laid a foundation for the dcvclopment of the sheep NK-Lysin peptides as candidate antimicrobial agents.关键词
绵羊/抗菌肽/沙门氏菌/攻毒/治疗Key words
sheep/antimicrobial peptide/Salmonella/challenge/treatment分类
农业科技引用本文复制引用
杨霞,张殿卿,宋佳玮,肖盛中,盛金良,剡根强..合成绵羊抗菌肽NK-Lysin抗菌活性及其对雏鸡沙门氏菌攻毒的治疗效果研究[J].中国畜牧兽医,2017,44(9):2739-2746,8.基金项目
国家自然科学基金(31360589) （31360589）
兵团博士资金专项(2014BB016) （2014BB016）
人社部留学回国人员启动(RSLX201304) （RSLX201304）
