中国畜牧兽医2017，Vol.44Issue(9)：2761-2766,6.DOI:10.16431/j.cnki.1671-7236.2017.09.031
猪附红细胞体小鼠感染模型建立条件的优化
Optimization of Establishment Conditions of Mouse Model of Mycoplasma suis
摘要
Abstract
In order to explore the infection characteristics of Mycoplasma suis (M.suis),and optimize the establishment of infection model of M.suis,this research designed the infection test with different mice (A test),different pathogen forms (B test),frozen pathogens (C test),and studied on mice model of blood reinfection (D test).On the basis of a comprehensive assessment of each test in the blood of mice infection,the first infection time,the clinical symptoms and the average duration of infection,to analyze whether the different treatments and different pathogenic mice forms,cryopreservation of pathogen and the mouse model of blood infection had influence on the establishment of mouse model.PCR detection,electron microscope observation and specific gene fragment sequencing were used to determine whether the pathogen of mice infected with the model was consistent with that of M.suis.The results showed that the infection effect of the spleen group was the best in the A test.In the B test,the infection effect of the M.suis positive blood samples group was the best.In the C test,the infection effect of the non-frozen positive blood group was better than that of the cryopreserved group.In the D test,the positive model of pig blood samples and mice model positive blood group had no significant difference.The results of PCR detection,electron microscope observation and specific gene fragment sequencing showed that the pathogen of mouse model was identical with that of M.suis.The results showed that the infection condition of the mice infected with the mouse model was influenced by the different treatment of the mice,the different forms of pathogens and the cryopreservation of the pathogen,and the model of Kunming mice without spleen infected with M.suis positive blood samples was the best.关键词
猪附红细胞体/小鼠/感染模型Key words
Mycoplasma suis/mouse/infection model分类
农业科技引用本文复制引用
倪婷婷,伍生军,梁晚枫,许应天,吴金桐,薛书江..猪附红细胞体小鼠感染模型建立条件的优化[J].中国畜牧兽医,2017,44(9):2761-2766,6.基金项目
国家自然科学基金(31460657) （31460657）
吉林省科技厅青年科研基金(20150520129JH) （20150520129JH）
