中国畜牧兽医2017，Vol.44Issue(9)：2783-2791,9.DOI:10.16431/j.cnki.1671-7236.2017.09.034
不同毒力水貂阿留申病毒在猫肾细胞中的增殖特性及凋亡特性的比较研究
Comparative Study of Proliferation and Apoptosis Characteristics of Different Virulence of Aleutian Mink Disease Virus in CRFK Cells
摘要
Abstract
This study was aimed to compare the proliferation and apoptosis characteristics of different virulence of Aleutian mink disease virus(AMDV) in feline kidney cell (CPFK).CRFK cells were infected separately with the standard strain of AMDV-G and the wild strains of AMDV-DL124,AMDV-DL125,AMDV-QD2,AMDV-QD3 and AMDV-ZJ3.Indirect immunofluorescence,Real-time quantitative PCR and TCID50 were used to detect the replication and expression of the viruses in the cells,at the same time the apoptosis induced by the viruses was detected.The results of IFA showed that CRFK cells which infected separately by wild strains appeared the fluorescence after 12 h of infection.With the extension of the infection time,the fluorescence of cells increased,but the fluorescence of AMDV-G was later than the wild strains,almost all of the cells were appeared the fluorescence after 72 h of infection.The Real-time quantitative PCR results showed that the tendency of genome replication of different virus were approximately the same.The genome replication began at 3 h after the AMDV-DL125 infection,the rapid increase of genome replication of AMDV-G occurred at 24 h after the infection,the genome replication of whole viruses reached a peak after 72 h of infection.The results of TCID50 showed that the latent period of viruses infection were 0 to 12 h after infection,AMDV-G reached the peak after 60 h of infection.However,the wild strains at 48 to 72 h could maintain a higher infection titer and reached peak at 72 h after infection,then decreased with the cell disintegration.Apoptosis detection results which analysis by SPSS 23.0 statistical analysis software showed that,compared with control group,cell apoptosis was significantly higher after 2 to 12 h of cells infected by wild strains induced (P＜0.05),cell apoptosis of AMDV-G was significantly lower than wild strains,however,the time of cell apoptosis induced by AMDV-G was longer,the apoptosis was still significant after 24 h of infection.But the apoptosis caused by virus infection was mostly concentrated in 2 to 12 h after infection.The results would provide some references for the study on the culture and identification and pathogenic mechanism of AMDV.关键词
水貂阿留申病毒(AMDV)/猫肾细胞(CPFK)/增殖特性/凋亡特性Key words
Aleutian mink disease virus (AMDV)/feline kidney cell (CPFK)/proliferation characteristics/apoptosis characteristics分类
医药卫生引用本文复制引用
王心舞,冷雪,杜锐..不同毒力水貂阿留申病毒在猫肾细胞中的增殖特性及凋亡特性的比较研究[J].中国畜牧兽医,2017,44(9):2783-2791,9.基金项目
产业创新战略联盟项目(20140309018YY) （20140309018YY）
吉林省科技厅科技成果转化促进计划(20140412009XH) （20140412009XH）
