中国畜牧兽医2017，Vol.44Issue(9)：2792-2800,9.DOI:10.16431/j.cnki.1671-7236.2017.09.035
鸡白痢沙门氏菌病原的分离鉴定及耐药性分析
Isolation, Identification and Analysis of Drug Resistance of Salmonella Pullorum
摘要
Abstract
In order to investigate the epidemic situation and drug resistance of major epidemic Salmonella Pullorum in some areas of Xinjiang,750 chickens with disease history were detected using the methods of plate agglutination,and a total of 78 cases were diagnosed infection with Salmonella Pullorumin by using Salmonella identification medium,histopathological observation,PCR and drug sensitivity test.The results showed that 69 of 78 cases suspect Salmonella Pullorum were isolated,and prove the biochemical characteristics and pathologic characteristics of Salmonella Pullorum,the invA gene was amplified successfully by PCR,the isolation rate was 9.2 ％ and the serotype was diagnosed as D1.The isolates were the most resistant to vancomycin and neomycin,and Salmonella Pullorum resistant rate was 100.0％ and 81.2％,respectively.Drug sensitive test results showed that the sensitive rate of the bacteria were more than 60.0 ％ to ceftriaxone,clavulanate and polymyxin.This study provided reference for farmers and veterinary workers,which indicated it could be used for the purification of Salmonella Pullorum and clinical medication.关键词
鸡白痢沙门氏菌/分离鉴定/组织病理学/PCR/药敏试验Key words
Salmonella Pullorum/isolation and identification/histopathology/PCR/drug sensitivity test分类
农业科技引用本文复制引用
刘志科,陈创夫,杨宁宁,徐锦凤,徐明国,荆明龙,吴文星,曹旭东,任艳,石峰..鸡白痢沙门氏菌病原的分离鉴定及耐药性分析[J].中国畜牧兽医,2017,44(9):2792-2800,9.基金项目
西部地区高发人兽共患传染性疾病防治协同创新项目(2013-179) （2013-179）
新疆重要人畜共患病分子疾病诊断机理研究项目(500003121002) （500003121002）
