中国畜牧兽医2017，Vol.44Issue(9)：2809-2815,7.DOI:10.16431/j.cnki.1671-7236.2017.09.037
PPARγ对猪血管内皮细胞增殖、迁移及小管形成的影响
Effect of PPAR γ on Proliferation, Migration and Tube Formation of Swine Vein Endothelial Cells
摘要
Abstract
In order to investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on proliferation,migration and tube formation of swine vein endothelial cells,and explore its role in swine placental angiogenesis,PPARγ agonist group (5,10,15 and 20 μmol/L rosiglitazone),inhibitor group (5,10,15 and 20 μmol/L T0070907) and control group were set in this study.Through iCelligence cell function analyzer,scratch test,Matrigel matrix three dimensional cell culture,a model of angiogenesis of swine vein endothelial cells in vitro was established for simulating the environment of pig angiogenesis in vivo and detecting the proliferation,migration,tube formation ability of cells.A pair of specific primer was designed by Primer Premier 5.0 software according to PPARγ sequence from NCBI.SYBR Green Ⅰ Real-time PCR were performed to detect relative quantity of PPARγ gene mRNA and verify the effect of PPARγ on cells in vitro.The results showed that 5 and 10 μ mol/L rosiglitazone could promote proliferation,migration and tube formation of swine vein endothelial cells,T0070907 had inhibition on cells and negatively correlated with concentration (5 to 15 μmol/L),both of rosiglitazone and T0070907 at high concentration (20 μmol/L) interfere in cells activity because of toxic effect.In addition,rosiglitazone (5 to 20 μmol/L) and T0070907 (5 to 20 μmol/L) increased and reduced relative quantity of PPARγ gene mRNA,respectively,and its trends of concentration were similar with the former three experiment.The results indicated that the activation of PPARγγpromoted proliferation,migration and tube formation of swine vein endothelial cells,and it might play a positive role in swine placental angiogenesis.关键词
过氧化物酶体增殖物激活受体γ(PPARγ)/猪/血管内皮细胞Key words
PPARγ/swine/vein endothelial cells分类
农业科技引用本文复制引用
彭璇,薛立群,张居作,袁安文..PPARγ对猪血管内皮细胞增殖、迁移及小管形成的影响[J].中国畜牧兽医,2017,44(9):2809-2815,7.基金项目
国家自然科学基金项目(31172377) （31172377）
