中国烟草学报2017，Vol.23Issue(5)：112-117,6.DOI:10.16472/j.chinatobacco.2017.121
过表达TaGS1/TaGS2对烟草抗盐能力的影响及其机制
Effects of overexpressing TaGS1/TaGS2 on tobacco salt tolerance and its mechanism
摘要
Abstract
In order to clarify that the increase of glutamine synthetase (GS) activity can improve salt tolerance in tobacco, this paper took cultivar tobacco K326 as control and studied TaGS1/TaGS2 transgenic tobacco salt tolerance and its mechanism. Results showed that under salt stress, TaGS1/ TaGS2 transgenic tobaccos, especially TaGS2 transgenic tobacco, had stronger root system and higher biomass than K326, and its ability to resist salt was superior to K326. TaGS1/TaGS2 transgenic tobacco had better carbon and nitrogen metabolism with higher chlorophyll content, stomatal conductance, net photosynthetic rate, GS activity, and soluble protein content. Content of proline and water of TaGS1/TaGS2 transgenic tobaccos were higher than that of K326, while content MDA of TaGS1/ TaGS2 transgenic tobaccos were lower than that of K326. Hence TaGS transgenic tobacco had stronger leaf osmotic regulation ability and plasma membrane protection ability. It was concluded that overexpression of TaGS1/TaGS2 could improve salt tolerance in tobacco, and its higher GS activity was the physiological basis to maintain high carbon and nitrogen metabolism and resistance to salt stress.关键词
烟草/GS/转基因/耐盐性Key words
tobacco/GS/transgene/salt tolerance引用本文复制引用
贾喜婷,韦一昊,谷明鑫,石爱博,王小纯..过表达TaGS1/TaGS2对烟草抗盐能力的影响及其机制[J].中国烟草学报,2017,23(5):112-117,6.基金项目
国家重点研发计划支持项目 (2016YFD0300205) （2016YFD0300205）
河南省现代农业产业技术体系 (S2010-01-G04) （S2010-01-G04）
