中国药房2017，Vol.28Issue(31)：4354-4358,5.DOI:10.6039/j.issn.1001-0408.2017.31.09
大黄与黄芪对慢性肾衰大鼠的肾保护作用及肠道屏障功能的影响
Effects of Rhei Radix and Astragali Radix on Renal Protection and Intestinal Barrier Function in Rats with Chronic Renal Failure
摘要
Abstract
OBJECTIVE:To study the effects of rhei radix and astragali radix on renal protection and intestinal barrier function in rats with chronic renal failure(CRF),and analyze its pharmacological mechanism from the theory of"gut-renal axis"in TCM. METHODS:Rat models of CRF were established by 5/6 nephrectomy. Rats were randomly divided into sham operations group (group J,n=6),model group(group M,n=8),and administration groups(n=9),which included benazepril group(group Y, positive control,2 mg/kg),rhei radix high-dose,medium-dose,low-dose groups(group DH,DM,DL,calculated by crude drug as 3,1.5,0.75 g/kg),and astragali radix high-dose,medium-dose,low-dose groups (group HH,HM,HL,calculated by crude drug as 6,3,1.5 g/kg). After 1 week of modeling,rats in each administration group were intragastrically administrated,once a day,for 5 weeks. Rats in group J,M were intragastrically administrated equal volume distilled water. After 12 h of administration, the 24 h urine protein(Upr),serum levels of creatinine(SCr),urea nitrogen(BUN),uric acid(UA)and plasma endotoxin level were detected,and kidney index was calculated. Changes in renal tissue,villi of small intestinal mucosal tissue and crypt depth were observed by microscope. RESULTS:Compared with group J,24 h urine volume and 24 h water drinking volume of rats in group M were increased,24 h Upr,serum levels of SCr,BUN,UA,plasma endotoxin level,and kidney index were increased, with statistical significances(P<0.05 or P<0.01). Most glomerular volume were increased and rigid in group M,showing intersti-tial infiltration in large number of inflammatory cells;villus length,villus width,mucosal thickness and villus length/crypt depth ra-tio of intestinal mucosa were decreased (P<0.05 or P<0.01),and crypt depth was increased (P<0.01). Compared with group M,24 h urine volume in group DM,DL,HM,HL was decreased;24 h water drinking volume in group HM was decreased;24 h Upr,serum levels of SCr,BUN levels,and kidney index of rats in each administration group were decreased. Except for group HH,serum UA levels in other administration groups was decreased;and except for group HH,HM,HL,plasma endotoxin level in other administration groups was decreased,with statistical significances(P<0.05 or P<0.01). The pathological morphologies of renal tissue,intestinal mucosal tissue and intestinal villi in each administration group were improved to varying degrees. CONCLU-SIONS:Both rhei radix and astragali radix can effectively reduce the metabolic toxin levels of rats,improve filtration function of glomerular and enhance the exclusion of metabolic trash,which may be restoring the intestinal function to achieve the goal of treat-ing CRF. And both show optimal effect in medium dose.关键词
大黄/黄芪/慢性肾衰/肠-肾轴/肠道屏障/大鼠Key words
Rhei radix/Astragali radix/Chronic kidney disease/Gut-renal axis/Intestinal barrier/Rats分类
医药卫生引用本文复制引用
涂祎珺,李海燕,宫仁豪,钟瑜萍,赖有勤..大黄与黄芪对慢性肾衰大鼠的肾保护作用及肠道屏障功能的影响[J].中国药房,2017,28(31):4354-4358,5.基金项目
国家自然科学基金青年基金项目(No.81403122) （No.81403122）
