中国药房2017，Vol.28Issue(31)：4381-4383,3.DOI:10.6039/j.issn.1001-0408.2017.31.16
UPLC法测定大鼠血浆中那格列奈的浓度及药动学研究
Concentration Determination of Nateglinide in Rats'Plasma by UPLC and Study on Its Pharmacokinetics
摘要
Abstract
OBJECTIVE:To establish a method for the concentration determination of nateglinide in rats'plasma and study its pharmacokinetic characteristics in rats in vivo. METHODS:UPLC was performed on the column of Acquity UPLC? BEH C18 with mobile phase of acetonitrile-10 mmol/L potassium dihydrogen phosphate buffer(41:59,V/V)at flow rate of 0.38 mL/min,with col-umn temperature of 35 ℃,detection wavelength of 210 nm and volume of 2 μL. 18 Wister rats were intragastrically administrated nateglinide 16 mg/kg. Blood sample 0.4 mL was taken from medial canthus before administration and after 10,20,30,45,60, 90,120,180,240,360,480 min of administration. The concentration of nateglinide in rats'plasma was determined;then DAS 2.1.1 software was used to calculate its pharmacokinetic parameters. RESULTS:Nateglinide showed good linear relationship in 0.05-6.4μg/mL(r=0.9993),lower limit of quantification was 0.05μg/mL;RSDs of inter-day(n=5),intra-day(n=3)and sta-bility (n=3) tests were lower than 10%;extraction recovery rate and method recovery rate were 78.71%-80.56%,91.78%-100.42%(RSD<10%,n=5),respectively. After rats were intragastrically administrated nateglinide,AUC0-8 h was (5.87 ± 2.32)μg·h/mL,AUC0-∞was(6.11±2.48)μg·h/mL,t1/2 was(1.72±0.55)h,tmax was(0.67±0.29)h and cmax was(3.34±1.23)μg/mL. CONCLUSIONS:The method is accurate,rapid with strong specificity,and can be used for the concentration determination of nat-eglinide in rats in vivo;nateglinide is absorbed and metabolized quickly in rats in vivo.关键词
那格列奈/超高效液相色谱法/药动学/大鼠Key words
Nateglinide/UPLC/Pharmacokinetics/Rats分类
医药卫生引用本文复制引用
王凯,蒋大鹏,张伟,王淑梅..UPLC法测定大鼠血浆中那格列奈的浓度及药动学研究[J].中国药房,2017,28(31):4381-4383,3.基金项目
河北省药学会2015年度临床药学专项科研项目(No.YX201505) （No.YX201505）
