中国药理学通报2017，Vol.33Issue(11)：1535-1541,7.DOI:10.3969/j.issn.1001-1978.2017.11.012
果寡糖对水飞蓟宾治疗非酒精性脂肪肝的增效作用研究
Modulation of fructo-oligosaccharide during silybin-meditated improvement of nonalcoholic fatty liver
摘要
Abstract
Aim To examine weather fructo-oligosaccharides(FOS) and silybin(Sil) could exhibit synergetic effect on treating obesity-associated non-alcoholic fatty liver disease(NAFLD).Methods Seventy mice were randomly divided into two groups:control group (NCD,fed with normal chow diet),the other sixty mice were fed with high-fat diet (HFD) to establish NAFLD model.Seventy days after the establishment of experimental model,the latter group was then randomly subdivided into six groups:model (HFD),Sil (30mg· kg-1),FOS (2 000 mg · kg-1),Sil (30 mg ·kg-1) combined with FOS of high,medium and low dose respectively.The NCD and HFD group were given 0.5％ CMC,and the other groups were fed with high-fat diet and given 10 mL · kg-1 by gavage daily,then body weight and food intake were recorded.Fasting blood glucose,insulin,homeostasis model of assessment for insulin resistence index (HOMA-IR) and oral glucose tolerance tests(OGTT) were measured after 120 days.All mice were sacrificed after 130 days,and blood and liver were collected.Levels of TC,TG,ALT,AST in serum were detected,and liver index and pathology were also examined.Results FOS (2 000 mg · kg-1) showed obvious synergism for Sil-mediated attenuation of levels of TC,TG,ALT,AST in serum,fasting blood glucose,insulin,HOMA-IR,OGTI curve,liver index and pathology,but FOS (4 000 mg · kg-1) could not bring superiority with a double dose,except for its improvement in body weight of mice with NAFLD.Conclusions In the treatment of NAFLD,FOS exhibits synergetic effect with Sil.This agent might be a potent candidate for obesity and NAFLD prevention,through modulating the composition of gut microbiota.关键词
果寡糖/水飞蓟宾/非酒精性脂肪肝/增效/高脂饮食/肠道微生物Key words
fructo-oligosaccharide/silybin/non-alcoholic fatty liver disease/synergetic effect/high-fat diet/gut microbiota分类
医药卫生引用本文复制引用
李秀侠,文雪华,王燕平,吴萍,徐寅生..果寡糖对水飞蓟宾治疗非酒精性脂肪肝的增效作用研究[J].中国药理学通报,2017,33(11):1535-1541,7.基金项目
国家自然科学基金资助项目(No 81402987) （No 81402987）
