中国药理学通报2017，Vol.33Issue(11)：1541-1545,5.DOI:10.3969/j.issn.1001-1978.2017.11.013
芍药苷抑制肌球蛋白轻链激酶缓解肠上皮细胞屏障功能紊乱
Paeoniflorin alleviates TNF-α induced intestinal barrier dysfunction by inhibition of MLCK
摘要
Abstract
Aim To investigate the protective effect of paeoniflorin on TNF-α induced intestinal epithelial barrier dysfunction and its mechanisms.Methods The Caco-2 cells were cultured and the MTF assay was used to determine the effects of the paeoniflorin on Caco-2 cell activity.The Caco-2 cell intestinal epithelial barrier dysfunciton model was established through incubation of cells with TNF-α.The effects of paeoniflorin on intestinal epithelial barrier dysfunciton were studied.Results The transmembrane resistance in Caco-2 epithelial barrier was significantly reduced by TNF-α incubation;MLCK significantly increased,while tight junction protein occludin and ZO-1 significantly decreased by TNF-α.These changes were significantly reversed by paeoniflorin,which reduced MLCK expression and enhanced expression of occludin and ZO-1.The protective effects against epithelial barrier dysfunction could be abrogated by small interfering RNA(siRNA) of MLCK.Conclusions Paeoniflorin alleviates the epithelial barrier dysfunction induced by TNF-αthrough down-regulation of MLCK and enhancement of tight junction protein occludin and ZO-1.This study supplies a potential candidate drug for the clinical treatment of inflammatory bowel diseases.关键词
溃疡性结肠炎/芍药苷/肿瘤坏死因子α/Caco-2细胞/肠上皮屏障/肌球蛋白轻链激酶Key words
ulcerative colitis/paeoniflorin/tumor necrosis factor alpha/Caco-2 cells/intestinal epithelial barrier/myosin light chain kinase分类
医药卫生引用本文复制引用
王亮,杨芳,回斯美,周子娟,陈军,詹红微,陈大朋,王靖宇..芍药苷抑制肌球蛋白轻链激酶缓解肠上皮细胞屏障功能紊乱[J].中国药理学通报,2017,33(11):1541-1545,5.基金项目
国家自然科学基金资助项目(No 81600440,31272392) （No 81600440,31272392）
