中国药理学通报2017，Vol.33Issue(11)：1546-1552,7.DOI:10.3969/j.issn.1001-1978.2017.11.014
海蛾活性部位抑制人宫颈癌HeLa细胞增殖及诱导凋亡作用研究
Effects of active fraction from Pegasus laternarius Cuvier on proliferation and apoptosis of human cervical cancer HeLa cells
摘要
Abstract
Aim To isolate HeLa cell proliferation-inhibitory active fraction from Pegasus laternarius Cuvier and explore its potential apoptosis-inducing mechanism.Methods To obtain the active fraction,the ethanol extract of Pegasus laternarius Cuvier was chromatographed by silica gel and sephadex LH-20 columns;MTT assay was used to evaluate the proliferation-inhibitory ability of active fraction on HeLa cells;AO/EB,PI and Annexin V-FITC/PI fluorescent staining flow cytometry were used to evaluate its apoptosis-inducing ability;the possible mechanism was investigated by analyzing the enzyme activity of caspase-3 and the protein expression of apoptosis-related genes in tumor cells.Results A fraction of C22 with high HeLa proliferation-inhibitory activity was isolated,with a yield of 0.73 ‰ and an IC50 of 36.3 mg · L-1;fraction C22 could increase the proportion of cells in sub-G0/G1 phase,phosphatidylserine eversion and other typical cell apoptosis in a dose-dependent manner;fraction C22 could down-regulate the expression of Bcl-2 and increase the enzyme of caspase-3 in HeLa cells.Conclusions The active fraction C22 from Pegasus laternarius Cuvier can inhibit the proliferation of HeLa cells by inducing apoptosis.The effect of inducing apoptosis may be conducted through mediating the mitochondrial Bcl2/caspase pathway.关键词
海蛾/HeLa细胞/生物活性追踪法/诱导凋亡/流式细胞术/Bcl-2/caspase-3Key words
Pegasus laternarius Cuvier/HeLa cells/bioassay-guided method/apoptosis-inducing/flow cytometry/Bcl-2/caspase-3分类
医药卫生引用本文复制引用
李锋,裴世锋,石贤爱..海蛾活性部位抑制人宫颈癌HeLa细胞增殖及诱导凋亡作用研究[J].中国药理学通报,2017,33(11):1546-1552,7.基金项目
福建省自然科学基金资助项目(No 2017J01580) （No 2017J01580）
