中国药理学通报2017，Vol.33Issue(11)：1584-1588,5.DOI:10.3969/j.issn.1001-1978.2017.11.021
表没食子儿茶素没食子酸酯对心肌细胞缺氧/复氧损伤的抑制作用及其机制
Protective effect of epigallocatechin-3-gallate on hypoxia/reoxygenation injury in cardiac myocytes and its mechanism
摘要
Abstract
Aim To observe the protective effect of epigallocatechin-3-gallate (EGCG) on hypoxia/reoxygenation (H/R) injury of cardiac myocytes and its mechanisms.Methods H9c2 cardiac myocytes were cultured in vitro and randomly divided into five groups:normal group(N group),H/R group,EGCG low dose group (L group),EGCG medium dose group (M group),and EGCG high dose group(H group).The cardiomyocyte H/R injury model was established and EGCG was pretreated.Cell survival rate was tested by CCK-8 method.The cell apoptotic rate was detected using Annexin V-FITC/PI double staining.The contents of total antioxidant capacity(T-AOC) and tumor necrosis factor α(TNF-α) in cell culture medium were tested according to the kit instructions.The protein expression of Akt and p-Akt was observed using Western blot,while the gene expressions of PI3K,Akt,caspase3 were detected by using fluorescence quantitative PCR method.Results Compared with model group,EGCG increased cell survival rate and reduced the apoptosis after H/R injury.Meanwhile,pretreatment EGCG improved the activity of T-AOC,reduced the level of TNF-α,up-regulated the expression of PI3K,Akt and p-Akt,and down-regulated the expression of caspase3.Conclusion EGCG reduces apoptosis and protects cardiac myocytes by influencing PI3K/Akt signal path关键词
EGCG/H9c2心肌细胞/缺氧/复氧损伤/细胞凋亡/PI3K/Akt/caspase-3Key words
EGCG/H9c2 cardiac myocytes/hypoxia/reoxygenation injury/cell apoptosis/PI3K/Akt/caspase-3分类
医药卫生引用本文复制引用
郑晓佳,余婷,张华,李春凤,陈叶霞,阳勇珍,李晨昕,简洁..表没食子儿茶素没食子酸酯对心肌细胞缺氧/复氧损伤的抑制作用及其机制[J].中国药理学通报,2017,33(11):1584-1588,5.基金项目
广西高校大学生创新创业计划项目(No 201510601007) （No 201510601007）
广西高等学校优秀人才资助计划项目 （）
