中国药理学通报2017，Vol.33Issue(11)：1596-1600,5.DOI:10.3969/j.issn.1001-1978.2017.11.023
载天冬酰胺酶磺丁基-β-环糊精脂质体的稳定性及相关机制
Stabilities and related mechanism of sulfobutyl ether-β-cyclodextrin liposomes loaded with asparaginase
摘要
Abstract
Aim To investigate the stabilities and related mechanism of sulfobutyl ether-β-cyclodextrin liposomes loaded with asparaginase(ASDL).Methods Reverse evaporating method was used for the preparation of ASDL,and the acid-base stability,thermal stability,antitrypsin stability,plasma stability and storage stability of ASDL were tested.Moreover,fluorescence spectrum method was utilized to explore the stability enhancement mechanisms of ASDL.Results The results of stability showed that the acid-base stability,thermal stability,antitrypsin stability,plasma stability and storage stability of ASDL were all superior to asparaginase.Fluorescence experiment results indicated that the improved characteristics of ASDL might be related to the changes of the surrounding microenvironment of fluorescent chromophore or the structure of hydrophobic.Conclusion ASDL can effectively protect asparaginase from the external environment,such as hydrolase,pH,temperature and so on,to improve the stabilities of asparaginase.关键词
生物大分子/天冬酰胺酶/环糊精脂质体/稳定性/体外/荧光Key words
biomacromolecule/asparaginase/cyclodextrin liposomes/stabilities/in vitro/fluorescence分类
医药卫生引用本文复制引用
李瑶,万胜利,张永红,胡雪原,张景勍..载天冬酰胺酶磺丁基-β-环糊精脂质体的稳定性及相关机制[J].中国药理学通报,2017,33(11):1596-1600,5.基金项目
重庆市研究生科研创新项目(No CYS16133) （No CYS16133）
