中国药理学通报2017，Vol.33Issue(11)：1600-1605,6.DOI:10.3969/j.issn.1001-1978.2017.11.024
木蝴蝶挥发性成分对H22荷瘤小鼠实体瘤抑制作用及其机制初步研究
Effects of volatile components in Oroxyli Semen on H22 bearing mice and its possible mechanisms
摘要
Abstract
Aim To study the inhibitory effect of volatile components in Oroxyli Semen on liver cancer and its possible mechanisms.Methods H22 bearing mouse model was used,the mice were divided into six groups:blank,model,positive (cytoxan,100 mg · kg-1),low-,mid-,and high-dose (17.5,35,and 70 mg · kg-1) volatile components groups,and then the mice were ig given once daily for consecutive 12 d.Then the tumor growth inhibitory rate,spleen and thymus indexes were calculated;the serum levels of IL-2,IL-6 were determined.HE staining was used to study of the apoptosis of the solid tumor.After treatment of SMMC-7721 cells with 0 ～ 1 g · L-1 of volatile components for 24,48 and 72 h,MTT assay was used to examine the proliferation.TUNEL method was applied to detect cell apoptosis,and RT-PCR method to detect Bax,Bcl-2,caspase-3 mRNA experssion.Results The inhibitory rate of volatile components high-dose on H22 bearing mice was 42.08％.The thymus index and the contents of serum IL-2 and IL-6 of H22 bearing mice were significantly higher than those in model group.Volatile components could significantly inhibit proliferation and induce apoptosis of SMMC-7721 cells,downregulate the expression of Bcl-2 mRNA,and up-regulate the expression of Bax,caspase-3 mRNA.Conclusions The volatile components in Oroxyli Semen have obvious anti-tumor activity in vitro and in vivo,and its mechanism may be related to enhancing immune system and promoting tumor cell apoptosis.关键词
木蝴蝶挥发性成分/抗肝肿瘤/H22荷瘤小鼠/SMMC-7721/凋亡/作用机制Key words
volatile components in Oroxyli Semen/anti-tumor/H22 bearing mice/SMMC-7721/apoptosis/mechanism分类
医药卫生引用本文复制引用
李楠楠,孟宪生,包永睿,王帅,李天骄..木蝴蝶挥发性成分对H22荷瘤小鼠实体瘤抑制作用及其机制初步研究[J].中国药理学通报,2017,33(11):1600-1605,6.基金项目
国家科技部“重大新药创制”科技重大专项(No 20132X09507005-004) （No 20132X09507005-004）
