中国药理学通报2017，Vol.33Issue(11)：1605-1610,6.DOI:10.3969/j.issn.1001-1978.2017.11.025
基于UHPLC/Q-TOF/MS分析羊耳菊有效组分的入血成分
Analysis of components in plasma from effective fractions of Inula cappa based on UHPLC/Q-TOF/MS
摘要
Abstract
Aim To analyze the main components of effective fractions of Inula cappa in rat plasma with UHPLC/Q-TOF/MS technology and serum pharmacochemistry theory.Methods After gavage administration with Inula cappa extracts,blood was collected from abdominal aorta,and the protein in plasma sample was precipitated by methanol.Eclipse Plus C18 RRHD (2.1 mm × 100 mm,1.8 μm) was used,with 0.1％ formic acid agueous solution (A)-0.1％ formic acid and acetonitrile (B) as the mobile phase for gradient elution,and the flow rate was 0.3 mL ·min-1 Detected at negative ion mode,and with the help of Metabolite Tools software from Bruker Corporation,components in plasma were defined.Results A total of 16 compounds were identified,including 9 prototypes and 7 metabolites.Main metabolites were isomerization,methylation,glucuronidation and recombination of caffeoylquinic acid.Conclusions Therefore,our study has comprehensively expounded Inula cappa extracts' constituents migrating to rat plasma,and provided a reference for further studies for in vivo metabolic process and effective material base of Inula cappa.关键词
羊耳菊/血浆/入血成分/UHPLC/Q-TOF/MS/血清药物化学/代谢物Key words
Inula cappa/plasma/effective fraction/UHPLC/Q-TOF/MS/serum pharmacochemistry/metabolites分类
医药卫生引用本文复制引用
巩仔鹏,陈亭亭,侯靖宇,吴林霖,李梅,李月婷,陈思颖,兰燕宇..基于UHPLC/Q-TOF/MS分析羊耳菊有效组分的入血成分[J].中国药理学通报,2017,33(11):1605-1610,6.基金项目
国家自然科学基金资助项目(No 81360680) （No 81360680）
贵州省优青项目[黔科合人字(2015)11号] （2015）
贵州省教育厅“民族药与中药开发应用产学研基地建设”项目(黔科合KY字[2013]122) （黔科合KY字[2013]122）
贵州省科学技术基金项目[黔科合LH字(2015)7362] （2015）
贵州省研究生卓越人才计划项目(黔教研合ZYRC字[2014]012) （黔教研合ZYRC字[2014]012）
贵州省中医药管理局项目(No QZZY-2015-078) （No QZZY-2015-078）
