中国油料作物学报2017，Vol.39Issue(5)：600-605,6.DOI:10.7505/j.issn.1007-9084.2017.05.003
花生花青素合成相关基因的表达与种皮颜色关系
Differential expression of genes associated with anthocyanin synthesis in peanut cultivars with different testa color
摘要
Abstract
The synthesis and accumulation of anthocyanin is related to testa color of peanut cultivars. To reveal the relationship between testa color and expression patterns of genes associated with anthocyanin synthesis in pea-nut,four peanut cultivars with different testa colors were selected to characterize the anthocyanin composition and expression patterns of seven genes (PAL,CHS,CHI,F3′H,DFR,ANS,3GT)related to the bio - synthesis of antho-cyanin at 5 pod developmental stages,by HPLC and real - time quantitative PCR. The results showed that anthocy-anin in peanut testa was consisted of delphinidin,cyanidin and pelargonidin. The color of peanut testa had positive-ly correlation with the content of three pigmentations. The color of peanut testa was significantly correlation with high expression levels of chalcone synthase (CHS),chalcone isomerase (CHI),dihydroflavonol - 4 reductase (DFR),flavonoid 3'- ydroxylase(F3′H)and anthocyanidin synthase (ANS)during the seed filling stage (40 -50d after flowering).关键词
花生品种/种皮颜色/花青素合成/相关基因/差异表达Key words
peanut/testa color/isoflavone synthesis/related genes/differential expression分类
农业科技引用本文复制引用
李海芬,邱金梅,陈小平,洪彦彬,梁炫强..花生花青素合成相关基因的表达与种皮颜色关系[J].中国油料作物学报,2017,39(5):600-605,6.基金项目
国家自然科学基金(31501246) （31501246）
广东省科技计划项目(2015B020231006 （）
2015A020209051 （）
2016B020201003) （）
