安徽农业大学学报2017，Vol.44Issue(5)：761-767,7.DOI:10.13610/j.cnki.1672-352x.20171017.008
SDE和HE-SPME应用于GC-MS分析香榧假种皮中挥发性成分的比较研究
A comparative study of SDE and HS-SPME methods for analyzing volatile components in aril of Torreya grandis cv.Merrilli
摘要
Abstract
The volatile substances in Torreya grandis aril were extracted using the simultaneous distillation and extraction (SDE) and headspace solid phase microextraction (HS-SPME) method.The quantitative analysis was carried out using gas chromatography-mass spectrometry (GC-MS) combined with the peak area,while the qualitative analysis was conducted using GC-MS combined with the retention index.The results showed that 96 volatile substances with a matched-degree up to 90％ were detected by the two extraction methods.Of which,57 substances were detected by SED and 39 were detected by HS-SPME.Among the detected substances,60 were alkenes and 12,9,3 and 12 were alcohols,carboxylic acids and esters,aldehydes and ketones,and other com pounds,respectively.The results suggested that HS-SPME had such advantages as simple pretreatrnent,short time and better repeatability,indicating it is suitable for a rapid determination of volatiles.However,the combination of the two methods could provide an overall picture of volatile substances in aril of Torreya grandis.关键词
香榧假种皮/挥发性成分/顶空固相微萃取/同时蒸馏萃取/保留指数Key words
Torreya grandis aril/volatile components/headspace solid-phase microextraction/simultaneous distillation extraction/retention index分类
轻工纺织引用本文复制引用
常慧,许睿洁,杨松,陈昭琪,亢金秀,杨文宇,罗静璠,丁之恩..SDE和HE-SPME应用于GC-MS分析香榧假种皮中挥发性成分的比较研究[J].安徽农业大学学报,2017,44(5):761-767,7.基金项目
国家自然科学基金(30271100)资助. （30271100）
