安徽农业大学学报2017，Vol.44Issue(5)：784-789,6.DOI:10.13610/j.cnki.1672-352x.20171017.002
安徽省克氏原螯虾白斑综合征病毒变异区序列分析
Sequence analysis of variable regions of white spot syndrome virus from Procambarus clarkii in Anhui Province
摘要
Abstract
White spot syndrome has caused significant economic loss in Procambarus clarkii industry in Anhui Province.To understand the molecular epidemiology of white spot syndrome virus (WSSV),we collected nine cultured P.clarkii samples between April and August 2016 from six cities in Anhui Province and performed nested-PCR analyses to detect WSSV and amplify the sequences of ORF94,ORF75 and ORF 125 variable regions.The results were as follows:nine samples were all WSSV positive in the first PCR amplification.The numbers of repeats units (RU) in ORF94 were 2,4,7,8 and 12,while 2 RUs was the dominant genotype.The numbers of compound RUs in ORF75 were 3,6,9,10 and 11.The most dominant genotype displayed 9 RUs with the positioning of 45,102,45,102,3×45,102 and 45.The RUs numbers of ORF125 ranged from 5 to 9,and the most prevalent genotypes were 7 RUs and 6 RUs.关键词
克氏原螯虾/白斑综合征病毒/ORF94/ORF75/ORF125/序列分析Key words
Procambarus clarkii/white spot syndrome virus/ORF94/ORF75/ORF125/sequence analysis分类
农业科技引用本文复制引用
陈红莲,王永杰,蒋业林,程云生,张静,王芬..安徽省克氏原螯虾白斑综合征病毒变异区序列分析[J].安徽农业大学学报,2017,44(5):784-789,6.基金项目
安徽省农科院学科建设项目(16A0516),安徽省农科院科技创新团队(14C0504) （16A0516）
安徽省农科院院士工作站(科人[2014]12)共同资助. （科人[2014]12）
