安徽农业科学2017，Vol.45Issue(33)：142-145,152,5.
盐碱土锈腐病拮抗菌的筛选和鉴定
Screening and Identification of Antagonistic Bacteria for Rust Rot from Saline-alkali Soil
摘要
Abstract
[Objective] Haloalkaliphilic bacterial strains were screened from saline-alkali soil in Daqing City, Heilongjiang Province.[ Meth-od] Strains were screened with plate dilution method on different NaCl concentration gradient Sehgal-Gibbons medium.The haloalkaliphilic bacteria with antagonistic activity against rust rot was obtained by plate confrontation method and identified based on morphological observation and modern molecular biology techniques.[Result] 48 strains of bacteria were isolated from saline-alkali soil in Daqing City, 60.4% of the strains were grouped into the halotolerant bacteria, and the rest into the moderate halophiles.Alkaline resistance of the strains was between pH 8.0 and 11.0, 64.6% of which pH tolerance reached to pH 11.0.The rest was alkali-resistant bacteria except two basophilic bacteria.3 strains were obtained by screening antagonistic activity of rust rot C.destructans R2.Morphology,physiological and biochemical characters and phylogeny of 16SrRan gene sequence were analyzed.3 strains were identified as Halomonas elongate,Halomonas xianhensis and Oceanobacillus iheyensis.[Conclusion] 3 haloalkaliphilic bacterial with antagonistic activity against rust rot were screened and identified from saline-alkali soil.关键词
盐碱土/拮抗菌/锈腐病/筛选/鉴定Key words
Saline-alkali soil/Antagonistic bacteria/Rust rot/Screening/Identification分类
农业科技引用本文复制引用
丁婷婷,杨泽斌,董广蕊,刘勇,贺香草,张鸿雁..盐碱土锈腐病拮抗菌的筛选和鉴定[J].安徽农业科学,2017,45(33):142-145,152,5.基金项目
黑龙江八一农垦大学大学生创新项目. （）
