安徽农业科学2017，Vol.45Issue(33)：146-148,3.
极早熟李品种"红美丽"的组织培养与快速繁殖
Tissue Culture and Rapid Propagation of Early Matured Plum Cultivar "Red Beauty"
摘要
Abstract
[Objective] To establish the system of tissue culture and rapid propagation of early matured plum cultivar"Red Beauty".[ Meth-od] Semi-lignified young shoots were selected as explants, the effects of basal medium composition on the initial growth of axillary bud were in-vestigated.In vitro shoots were selected as materials, the effects of basal medium composition and the concentration of plant growth regulators on proliferation of tube seedling and rooting of tube seedling were examined.[ Result] Basal medium QL evidently improved the sprouting per-centage of axillary buds in inducing the initial growth of axillary buds when half lignification stem section was selected as explant.For prolifera-tion, WPM was best, followed by MS and QL.For rooting percentage, there was no significant difference among different auxin concentra-tions.But the higher concentration of 2 mg/L IBA significantly improved the average root number per plantlet than lower concentration of 1 mg/L IBA.The best rooting medium was MS+2 mg/L IBA+0.5 mg/L NAA.Rooting percentage was 83.5%.[Conclusion] This research laid a research and technical foundation for virus-free plantlet production of plum cultivar"Red Beauty"and breed improvement by using bi-otechnology breeding methods.关键词
李/"红美丽"/组织培养/增殖/生根Key words
Plum/"Red Beauty"/Tissue culture/Proliferation/Rooting分类
农业科技引用本文复制引用
孙清荣,王金政,薛晓敏,关秋竹,孙洪雁..极早熟李品种"红美丽"的组织培养与快速繁殖[J].安徽农业科学,2017,45(33):146-148,3.基金项目
山东省农业良种工程项目. （）
