安徽医科大学学报2017，Vol.52Issue(9)：1261-1265,5.DOI:10.19405/j.cnki.issn1000-1492.2017.09.001
HIF-1α介导BMSCs复合PLGA修复大鼠颅骨标准骨缺损的研究
Effects of HIF-1α transfected BMSCs combined with PLGA on repairing rat calvarial bone defects
摘要
Abstract
Objective To construct the vascular tissue engineering bone of hypoxia-inducible factor-1 α (HIF-1α)mediated bone mesenchymal stem cells (BMSCs) combined with polylactic-co-glycolicacid (PLGA) scaffold,and to observe the effect on repairing the critical-sizedcalvarial bone defect in rat.Methods The target gene HIF-1 α was transfected into BMSCs,and combined with PLGA scaffold,then implanted to repair bilateralcalvarial defect (Φ 5 mm) of SD rat (n =18).The rats were randomly divided into three groups:HIF-1α-BMSCs/PLGA group (n =6),BMSCs/PLGA group (n =6) and only PLGA scaffold group (n =6).The new bone formation was observed by gross inspection,X-ray examination and HE staining respectively at 8 weeks after surgery.Results The gross inspection,X-ray examination and HE staining showed that the amount of new bone formation in the experimental group was significantly higher than that in the control group and the material group,and the difference was statistically significant (P ＜ 0.05).Conclusion HIF-1 can mediate BMSCs to promote bone tissue formation.PL-GA is an ideal scaffold material and the bone defect can be repaired effectively by using this vascular tissue engineering bone.关键词
低氧诱导因子-1α/骨髓间充质干细胞/聚乳酸-聚乙醇酸共聚物/组织工程骨/标准骨缺损Key words
hypoxia-inducible factor-1α/bone marrow mesenchymal stem cells/polylactic-co-glycolicacid/tissue-engineered bone/critical-sized bone defects分类
医药卫生引用本文复制引用
朱艳秋,王默涵,周咏,许敏,何家才..HIF-1α介导BMSCs复合PLGA修复大鼠颅骨标准骨缺损的研究[J].安徽医科大学学报,2017,52(9):1261-1265,5.基金项目
国家自然科学基金(编号:81371114) （编号:81371114）
安徽省自然科学基金(编号:1408085MKL29) （编号:1408085MKL29）
安徽省科技攻关项目(编号:1604a0802082) （编号:1604a0802082）
