安徽医科大学学报2017，Vol.52Issue(9)：1331-1335,5.DOI:10.19405/j.cnki.issn1000-1492.2017.09.017
大鼠ALPPS术后残肝再生与Wnt2蛋白表达关系的研究
Study on the relationship between remnant liver regeneration and Wnt2 protein expression in rats after ALPPS
摘要
Abstract
Objective To evaluate expression of WNT2 and its relationship with remnant liver regeneration after associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) in rats and the underling mechanisms in liver regeneration.Methods Forty healthy male Sprague-Dawley (SD) rats (200 ～240 g) were randomly divided two groups:ALPPS group,Sham group.The Sham group only freed vein branches without ligation and closed its abdomen.The ALPPS group consisted of 70％ portal vein ligation(occluding the left middle,left lateral,and right lobes) and partial(10％) hepatectomy(caudate lobe),parenchymal transaction(median lobe),the right middle lobe was preserved to regenerate.Five rats were sacrificed on day 1,2,4 and 7 in each group after operation to observe the weight of the right median lobe.The morphological changed of the right middle lobe liver lobes were observed by microscope.The expression of Wnt2 and Ki-67 of the right middle lobe liver lobes were studied with immunohistochemistry.The results were analyzed by medical statistics methods.Results ① Remnant liver regenerate after ALPPS in rats,liver regeneration increased the fastest at the second day,then the speed of liver regeneration rate decreased gradually,there was no statistical significance in ALPPS group compared with Sham at the seventh day;② Compared with the Sham group,the Ki-67 after surgery began to increase,it reached the maximum at the second day,then decreased gradually,there were a few positive cells at the seventh day and no statistical significance;③ The positive expression of Wnt2 was mainly around the central vein,it may be associated with the generation of liver stem cells.Compared with Sham group,Wnt2 in remnant liver began to rise after ALPPS,reached the highest at the second day,and then decreased gradually,only a small amount of expression at the seventh day and there was no statistical significance;④ The expression of Wnt2 and Ki-67 had a positive correlation after ALPPS.Conclusion The expression of Wnt2 may play a pivotal role in liver regeneration and liver stem cell after ALPPS.关键词
肝再生/ALPPS/Wnt2Key words
liver regeneration/ALPPS/Wnt2分类
医药卫生引用本文复制引用
刘伟伟,余锋,刘洪,李福利,柏杨,罗昆仑..大鼠ALPPS术后残肝再生与Wnt2蛋白表达关系的研究[J].安徽医科大学学报,2017,52(9):1331-1335,5.基金项目
南京军区医学科技创新课题重点资助项目(编号:14D05) （编号:14D05）
