安徽医科大学学报2017，Vol.52Issue(10)：1446-1449,4.DOI:10.19405/j.cnki.issn1000-1492.2017.10.007
白藜芦醇抑制低浓度H2O2处理佐剂性关节炎大鼠成纤维样滑膜细胞增殖的机制研究
Resveratrol inhibits low concentration of H2O2 treatment of fibroblast-like synoviocytes proliferation and the possible mechanism in adjuvant arthritis rats
摘要
Abstract
Objective To explore antioxidant resveratrol inhibits low concentration of H2 O2 treatment of fibroblastlike synoviocytes (FLS) proliferation and the possible mechanism in adjuvant arthritis (AA) rats.Methods Freund' s complete adjuvant (FCA) injected subcutaneously into SD rats induced by AA,the AA rats were sacrificed in 14th days,serum oxidative stress indicators were checked,different concentrations of H2O2 proliferation effect of FLS CCK8 was observed,oxidative stress related protein expression of SIRT3,MnSOD were detected by Western blot.Results Serum levels of oxidative stress indicators increased in AA model rats,in vitro experiments showed that with the concentration of Res increased,low concentration of H2O2-treated AA rats FLS proliferation was inhibited,SIRT3,MnSOD protein expression decreased.Conclusion Res can reduce the oxidative stress status in AA rats,and its mechanism is related to the decrease of anti-oxidative stress protein SIRT3 and MnSOD.关键词
佐剂性关节炎/成纤维样滑膜细胞/白藜芦醇/去乙酰化酶3/锰超氧化物歧化酶Key words
adjuvant arthritis/fibroblast-like synoviocytes/resveratrol/SIRT3/MnSOD分类
医药卫生引用本文复制引用
李娜,张俊强,曹威,殷瞳昕,胡永涛,陈晓宇..白藜芦醇抑制低浓度H2O2处理佐剂性关节炎大鼠成纤维样滑膜细胞增殖的机制研究[J].安徽医科大学学报,2017,52(10):1446-1449,4.基金项目
国家自然科学基金面上项目(编号:81373421) （编号:81373421）
安徽医科大学七年制临床医学专业学生“早期接触科研”训练计划项目(编号:2016-ZQKY-57) （编号:2016-ZQKY-57）
