安徽医科大学学报2017，Vol.52Issue(10)：1536-1539,4.DOI:10.19405/j.cnki.issn1000-1492.2017.10.027
安徽地区铜绿假单胞菌耐药性分析及泛耐药菌体外联合药敏试验的研究
Analysis of antibiotics resistance among Pseudomonas aeruginosa in Anhui Province and in vitro antimicrobial combinations for pan-drug resistant Pseudomonas aeruginosa
摘要
Abstract
Objective To investigate the distribution and antimicrobial resistance of Pseudomonas aeruginosa infection in Anhui Province,and collect all the pan-drug resistant Pseudomonas aeruginosa (PDRPA) using the microtiter plate chequerboard assay as a test in vitro bacteriostasis experiment,which providing reference for the choice of clinical medicine.Methods Minimal inhibitory concentration of Pseudomonas aeruginosa strains were tested by using agar dilution method.The resistance rate,the medium rate and the sensitivity rate of the antimicrobial agents were calculated according to the guidelines of the American Clinical Laboratory Committee guidelines.Picking out PDRPA,using the method of board joint drug susceptibility test in vitro bacteriostasis experiment,provide reference for the choice of clinical medicine.Results The resistances of PAE to amikacin,piperacillin/tazobactam,cefepime and ceftazidime were sensitive and stable;the resistances to ceftizoxime were significantly increased (P ＜ 0.05);the resistance to ciprofloxacin was decreased to some point(P ＜ 0.05);the resistances to imipenem and meropenem were stable.Polymyxin E + rifampicin,polymyxin E + imipenem,polymyxin E + cephalosporins,piperacillin/ tazobactam + amikacin,cephalosporins + ciprofloxacin,5 groups of antimicrobial agents for PDR pseudomonas aeruginosa major synergy together.Conclusion Pseudomonas aeruginosa infection is still a certain proportion of clinical infection.Thus,it is necessary to strengthen the surveillance of prevalence of PAE drug resistance so as to provide a reference for clinical therapy.The polymyxin E and other anti-infective drug combination can be used as the generic drug resistance of Pseudomonas aeruginosa infection treatment.关键词
铜绿假单胞菌/耐药性/药物敏感试验/联合用药Key words
Pseudomonas aeruginosa/drug resistance/bacterial resistance surveillance/conbination分类
医药卫生引用本文复制引用
卞婷婷,刘艳艳,叶英,李家斌..安徽地区铜绿假单胞菌耐药性分析及泛耐药菌体外联合药敏试验的研究[J].安徽医科大学学报,2017,52(10):1536-1539,4.基金项目
国家自然科学基金(编号:81172737) （编号:81172737）
