吉林大学学报（医学版）2017，Vol.43Issue(6)：1087-1091,前插2,6.DOI:10.13481/j.1671-587x.20170604
T-2毒素对人肝癌HepG2细胞的增殖抑制作用及促凋亡作用
Inhibitory effect of proliferation and promotion effect of apoptosis induction of T-2 toxin on human hepatocellular carcinoma HepG2 cells
摘要
Abstract
Objective: To investigate the inhibitory effect of T-2 toxin on proliferation of the human hepatocellular carcinoma HepG2 cells and its promotion effect of apoptosis.Methods:The HepG2 cells in the logarithmic phase were selected and divided into control group (without T-2 toxin)and experimental groups (given 0.25,2.50,25.00,250.00 and 2500.00 μg·L-1 T-2 toxin).After 24 h treatment,the morphology of cells was observed under inverted microscope;the inhibitory rate of proliferation of cells was determined by MTT assay;the cell cycle and apoptotic rate of cells were analyzed by flow cytometry;Hochest 33258 staining was used to observe the apoptotic morphology of cells;the activity of caspase-3 in HepG2 cells was detected.Results:After treated for 24 h,the inverted microscope observation results showed that the number of the cells in experimental groups was decreased significantly and the cells shrank and deformed.The MTT results showed that compared with control group,the inhibitory rates of proliferation of the cells in experimental groups were increased (P <0.01).The flow cytometry results showed that compared with control group, the percentage of the cells in SubG1 phase in experimental group was significantly increased,and the apoptotic rates of the cells in experimental groups were significantly increased.The Hoechest 33258 staining results showed that the chromatin condensation was observed in the cells in experimental groups,and the nuclei were dense and stained.Compared with control group,the activities of intracellular caspase-3 of the cells in experimental groups were significantly increased (P < 0.01 ). Conclusion:T-2 can inhibit the proliferation of human hepatocellular carcinoma HepG2 cells,and induce the apoptosis.关键词
T-2毒素/肝肿瘤/细胞凋亡/caspase-3Key words
T-2 toxin/liver neoplasms/apoptosis/caspase-3分类
医药卫生引用本文复制引用
朱文赫,柳玉,张家琦,李艳,刘微,董媛,刘磊,王会岩..T-2毒素对人肝癌HepG2细胞的增殖抑制作用及促凋亡作用[J].吉林大学学报（医学版）,2017,43(6):1087-1091,前插2,6.基金项目
国家科技部科技重大专项资助课题(2012ZX09103-301-003) （2012ZX09103-301-003）
吉林省教育厅科研型项目资助课题(吉教科合字[2015]第397号) （吉教科合字[2015]第397号）
