吉林大学学报（医学版）2017，Vol.43Issue(6)：1115-1120,6.DOI:10.13481/j.1671-587x.20170609
北五味子酸性多糖对阿尔茨海默病模型小鼠学习记忆能力的改善作用
Improvement of acidic polysaccharose of Schisandra Chinensis on learning and memory functions of Alzheimer's disease model mice
摘要
Abstract
Objective:To observe the improvement of acidic polysaccharose of Schisandrae Chinensis (SCP-A) on the learning and memory functions of Alzheimer's disease (AD)model mice induced by Aβ25-35 ,and to clarify the related mechanisms. Methods:A total of 75 male C57BL mice were randomly divided into control group (injected with saline in intracerebroventricular,given distilled water intragastrically),model group (injected with Aβ25-35 intracerebroventricularly,given distilled water intragastrically),and 5,10,20 mg·kg-1 SCP-A groups (injected with Aβ25-35 intracerebroventricularly,given SCP-A intragastrically) (n = 15).The agents were administered once daily for 14 d,in which Aβ25-35 was injected intracerebroventricularly on the 8th day.On the 15th day after administration,step-through test and Morris water maze test were used to detect the escape latency,number of errors,and time of finding platform,number of passing platforms,dwell time in target quadrant of the mice;Western blotting method was used to detected the levels of Tau,GSK-3β and their phosphorylated proteins in hippocampus tissue of the mice.Results:The step-through test results showed that compared with control group, the escape latency of the mice in model group was obviously shortened and the number of errors was significantly increased (P <0.01);compared with model group,the escape latencies of the mice in 10 and 20 mg·kg-1 SCP-A groups were significantly prolonged (P <0.01),the number of errors was significantly decreased (P <0.05 or P <0.01).The Morris water maze test results showed that compared with control group,the time of finding platform of the mice in model group was significantly prolonged,the number of passing platform and dwell time in target quadrant were significantly reduced (P <0.01);compared with model group,the time of finding platform of the mice in 10 and 20 mg· kg-1 SCP-A groups was significantly shortened (P < 0.01),and the number of passing platform and the dwell time in target quadrant were significantly increased (P <0.01).The Western blotting results showed that compared with model group,the expression levels of phosphorylated protein Tau Ser199,Tau Ser396 and Tau Ser404 in hippocampus of the mice in 20 mg·kg-1 SCP-A group were significantly decreased (P <0.05), and the expression level of GSK-3βwas significantly decreased (P <0.01);the expression level of phosphorylated protein GSK-3βTyr216 was significantly decreased,and the expression level of phosphorylated protein GSK-3βSer9 was significantly increased (P <0.05).Conclusion:SCP-A has an anti-AD effect,which is related to regulating the activity of GSK-3βto reduce the level of phosphorylated protein Tau in the hippocampus tissue of the mice.关键词
北五味子/酸性多糖/阿尔茨海默病/Tau蛋白/糖原合成酶激酶3Key words
Schisandra chinensis/acidic polysaccharides/Alzheimer's disease/Tau/GSK-3β.分类
医药卫生引用本文复制引用
李贺,刘聪,李宁,王春梅,敬舒,孙靖辉,孙红霞,张成义,陈建光..北五味子酸性多糖对阿尔茨海默病模型小鼠学习记忆能力的改善作用[J].吉林大学学报（医学版）,2017,43(6):1115-1120,6.基金项目
吉林省科技厅科研项目资助课题(20150101230JC,201603103YY) （20150101230JC,201603103YY）
吉林省吉林市科技计划项目资助课题(20166018,20163054) （20166018,20163054）
