吉林大学学报（医学版）2017，Vol.43Issue(6)：1121-1124,前插3,5.DOI:10.13481/j.1671-587x.20170610
卵巢功能调控相关蛋白在不同发育阶段卵泡中的表达及其意义
Expressions of ovarian function-related proteins in ovarian follicles in different development stages and their significances
摘要
Abstract
Objective:To detect the expressions of ovarian function-related proteins in the ovarian follicles in different development stages,and to explore their roles and significances in the regulation of follicular development and ovarian function. Methods:The ovarian tissue containing primordial,primary,secondary follicles,mature follicles, atretic follicles and corpus albicans of the woman in the childbearing age was selected. Immunohisto chemical analysis was used to detect the expressions of Glyoxal enzyme I (glyoxalase I),ubiquitin carboxy-terminal hydrolase L1 (UCH-L1),heat shock protein 27 (HSP27),serum amyloid P (SAP)proteins in the ovarian follicles in different stages.The expression intensities of these proteins were compared.Results:The expression intensities of Glyoxalase I and UCH-L1 in the granulosa cells and theca cells of secondary follicles and marure follicles were strong,which were higher than those in the cytoplasm of primordial and primary follicles.The expression intensities of HSP27 in the cytoplasm of primordial and primary follicles were strong,which were higher than those in the granulosa cells and theca cells of secondary follicles and marure follicles.The expression intensities of Glyoxalase I and HSP27 in the atretic follicles were strong,which were higher than that in the growth follicles. The expressions of SAP were positive in the primordial,primary follicles,secondary and mature follicles and the expression intensities were not different.The expressions of four proteins in the corpus albicans did not express. Conclusion:The high expression of UCH-L1 and low expression of HSP27 are associated with the mature and development of follicles;the high expressions of Glyoxalase I and HSP27 are associated with the follicular atresia and ovarian failure.关键词
乙二醛酶I/泛素羧基末端水解酶L1/热休克蛋白27/血清淀粉样蛋白P/卵泡发育Key words
glyoxal enzyme I/ubiquitin carboxy-terminal hydrolase L1/heat shock protein 27/serum amyloid p/follicle development分类
医药卫生引用本文复制引用
张利群,姚兰,潘袁,赵芸鹤,蔡知天..卵巢功能调控相关蛋白在不同发育阶段卵泡中的表达及其意义[J].吉林大学学报（医学版）,2017,43(6):1121-1124,前插3,5.基金项目
吉林省科技厅自然科学基金资助课题(20160101072JC) （20160101072JC）
