草业科学2017，Vol.34Issue(10)：2052-2062,11.DOI:10.11829/j.issn.1001-0629.2016-0580
利用EST-SSR标记构建中国老芒麦品种DNA指纹图谱及种质遗传多样性
EST-SSR marker based DNA fingerprinting for cultivar identification and genetic diversity analysis of Elymus sibiricus germplasm in China
摘要
Abstract
In this study,EST-SSR markers were used to analyze the genetic diversity of 52 Elynus sibiricus accessions,and to construct DNA fingerprinting profiles of seven E.sibiricus cultivated accessions and cultivars in China.A total of 204 bands were generated from 20 EST-SSR markers with an average of 10.2 bands per marker.Of the total,176 (86.27％) bands were polymorphic,indicating that the tested materials possessed a high level of polymorphism.The primers Elw404 and Elw195 could distinguish the seven cultivated accessions and cultivarsfrom other wild materials.Analysis of molecular variance showed that genetic variation was greater within geographical regions (73.94％) than between them (26.06％).Structure analysis suggested that the 52 accessions were clustered into five groups;a similar result was obtained using principal coordinate analysis.Compared with cultivars and cultivated accessions,wild accessions[number of polymorphic bands=174,percentage of polymorphic bands (PPB)=85.29％,Shannon's information index (Ⅰ)=0.296 6,Nei's genediversf ty (H)=0.179 8,observed number of alleles (Na)=1.852 9] possessed higher genetic diversity,suggesting that wild accessions could be used as important genetic resources for future breeding programs.Furthermore,ex situ and in situ conservation strategies were suggested for E.sibiricus germplasm.The results of the present study showed that EST-SSR markers provided an efficient means of assessing genetic diversity and identifying cultivars in E.sibiricus.关键词
老芒麦/DNA指纹图谱/EST-SSR/遗传变异/种质保护Key words
Elymus sibiricus/DNA fingerprinting/EST-SSR markers/genetic and variation/germplasm conservation分类
农业科技引用本文复制引用
张俊超,谢文刚,赵旭红,张宗瑜,赵永强,王彦荣..利用EST-SSR标记构建中国老芒麦品种DNA指纹图谱及种质遗传多样性[J].草业科学,2017,34(10):2052-2062,11.基金项目
国家基础研究项目(973项目)(2014CB138704) （973项目）
长江学者与创新团队发展计划项目(IRT-17R50) （IRT-17R50）
国家自然科学基金(31302023) （31302023）
中央高校基础研究基金(LZUJBKY-2016-7) （LZUJBKY-2016-7）
草地农业生态系统国家重点实验室开放课题 （）
