草业科学2017，Vol.34Issue(11)：2235-2244,10.DOI:10.11829/j.issn.1001-0629.2017-0049
葡萄风信子花青素3-O-葡糖基转移酶基因的克隆及其表达分析
Cloning and expression analysis of anthocyanidin 3-O -glucosyltransferase gene in Grape hyacinth
摘要
Abstract
Anthocyanidin 3-O -glucosyltransferase (3GT)is the last key enzyme in the anthocyanin biosynthetic pathway,which can catalyze unstable anthocyanidin into anthocyanin.In this study,we cloned a 3GT gene (designated as Ma3GT from Muscari armeniacum )using the PCR technique.The gene's cDNA was 1377 bp long and encoded an open reading frame of 458 aminoacids.Ma3GT showed 55%~64% similarity with the oth-er 3GTs.Phylogenetic analysis indicated that Ma3GT was classified into monocot subgroups of 3GTs,and had the closest relationship with the 3GTs of Freesia hybrid and Iris hollandica .QRT-PCR analysis detected tran-scripts of Ma3GT in different organs of M .armeniacum .The expression level of this gene was high in the fully opened petals (stage S4 ),very low in the leaves,and almost zero in the roots and bulbs of M .armeniacum . Andit almost had no expression in all the organs of M .aucheri 'White Beauty'and M .armeniacum 'Pink Surprise'.The gene expression was related to the floral developmental stages in M .armeniacum .The transcript level was rarely detectablein the unpigmented buds (stage S1 ),but increased during the pigment accumulation stages and peaked in the fully opened petals (S4 ).The expression had significant differences in three cultivars of different colors.The expression level was high in the petals of M .armeniacum ,and rarely detectable in the pet-als of 'White Beauty'and 'Pink Surprise.'This research will provide informationand theoretical support for the functional study of the Ma3GT gene.关键词
葡萄风信子/花青素苷/Ma3GT/RT-PCR/糖基转移酶/糖基化/花色Key words
Muscari/anthocyanin/Ma3GT gene/RT-PCR/glucosyltransferase/glycosylation/flower color分类
农业科技引用本文复制引用
杜灵娟,陈凯利,刘雅莉..葡萄风信子花青素3-O-葡糖基转移酶基因的克隆及其表达分析[J].草业科学,2017,34(11):2235-2244,10.基金项目
陕西省自然科学基金青年项目(2017JQ3019) （2017JQ3019）
中央高校基本科研业务费(Z109021603) （Z109021603）
国家自然科学基金青年项目(31700625) （31700625）
