草业科学2017，Vol.34Issue(11)：2245-2253,9.DOI:10.11829/j.issn.1001-0629.2016-0642
千日红无菌苗生长及试管开花诱导
Growth and in vitro flowering of Gomphrena globosa
摘要
Abstract
To clarify the mechanism of growth and in vitro flowering induction of Gomphrena globosa ,the shoots with apical bud were used as explants on modified Murashige and Skoog (MS)medium to explore the effects of different concentrations (0,20,40,60,or 80 g·L-1 )of sucrose,and different combinations of cy-tokinins [N6-benzyladenine (BA)and α-naphthaleneacetic acid (NAA)]and auxin polar transport inhibitors TIBA and NPA on nitrogen-regulated growth and flowering in vitro.The results indicated that sucrose was the key factor in floral morphogenesis and growth,and that treatments without sucrose (modified MS supplied with 6.5 g·L-1 agar)produced no flowers and few roots.An increasing concentration of sucrose could promote flowering,and the highest percentage of flowering (80.72%)was obtained on medium containing 80 g·L-1 sucrose.The height,leaf number,and particularly the root volume of G .globosa were all higher in treatments with 20~40 g·L-1 sucrose.L9 (34 )orthogonal experiment results showed that BA was more effective than NAA in promoting growth and flowering induction,with the highest percentage of flowering (60.14%)and growth being obtained on media containing 1.0 mg·L-1 BA.Auxin polar transport inhibitors prevented flower-ing and rooting of G .globosa ,with the inhibition effects of TIBA being stronger than those of NPA at the same concentrations,although auxin itself did not induce flowering.Therefore,sucrose is indispensable for the growth and in vitro flowering of G .globose .BA also plays an important role,and the effect was also regulated by endogenous hormones.关键词
千日红/试管开花/蔗糖/细胞分裂素(BA)/生长素(NAA)/生长素极性运输抑制剂Key words
Gomphrena globosa/flowering in vitro/sucrose/6-benzyladenine/α-naphthaleneacetic acid/auxin polar transport inhibitor分类
农业科技引用本文复制引用
张宵娟,邓光华,连芳青,邹娜,黄颖融,周树军..千日红无菌苗生长及试管开花诱导[J].草业科学,2017,34(11):2245-2253,9.基金项目
江西省教育厅青年基金(GJJ13253) （GJJ13253）
国家自然科学基金(31300521) （31300521）
