大豆科学2017，Vol.36Issue(6)：857-861,5.DOI:10.11861/j.issn.1000-9841.2017.06.0857
IFS转基因大豆的鉴定及高异黄酮植株的筛选
Identification of IFS Transgenic Soybean and Selection of Plant with High Isoflavones
摘要
Abstract
As a healthy active substances,soybean isoflavones have obvious effects in the prevention of cancer,osteoporosis,cardiovascular disease and women＇s climacteric syndrome.IFS is one of the key enzymes in the pathway of isoflavone biosynthesis and it had a positive effect on the content of isoflavones in soybean seeds,but we still haven＇t get stable and efficient transforms.Therefore,it is very important to improve the content of soybean isoflavones by studying isoflavone synthase pathway.In this study,after propagating IFS transgenic plants to T2 generation,physiological indexes in transgenic plants had no obviously changes by agronomic traits analyzing.Mter identifying IFS transgenic soybeans by RT-PCR analysis,there were 60 IFS transgenic soybeans in 125 plants,accounting for 48％.This result indicated that IFS was stablily inherited in offspring.We used the modified three wavelength method to measure the isoflavone content and results showed that the average isoflavone content was 1.2 mg·g-1 in IFS transgenic plants.Among the 41 IFS positive plants,isoflavone content of 15 individual plants were higher than that of the non-genetically modified plants,which accounted for 36.6％.The IFS had a certain effect on the improvement of isoflavone content and it is possible to select plants with high isoflavones in IFS transgenic soybeans.This study clarified the effect of IFS on the content of isoflavones in transgenic soybean and obtained transgenic soybean containing much soybean isoflavones and provided an excellent germplasm resource for soybean breeding at the meantime.In additionally,the improved three wavelength method was more accurate and faster.关键词
大豆/IFS/异黄酮含量/三波长法Key words
Soybean/IFS/Isoflavone content/Three wavelength method分类
农业科技引用本文复制引用
祁延萍,刘雅婧,许乐义,王凌妍,朴哲主,崔光赫,闫帆,王庆钰..IFS转基因大豆的鉴定及高异黄酮植株的筛选[J].大豆科学,2017,36(6):857-861,5.基金项目
吉林大学大学生创新创业训练计划(2015821253) （2015821253）
农业部转基因生物新品种培育重大专项(2016ZX08004003-004). （2016ZX08004003-004）
