大豆科学2017，Vol.36Issue(6)：862-865,4.DOI:10.11861/j.issn.1000-9841.2017.06.0862
大豆胞囊线虫病3号生理小种的抗性候选基因GmRSCN3-2的初步鉴定
Primary Identification of Candidate Gene GmRSCN3-2 in Soybean Cyst Nematode Race 3 Resistance
摘要
Abstract
The soybean cyst nematode (SCN) Race 3 was the prevalent race in Northeast China,which caused the serious yield loss.Presently,it is short of resistance cultivar in the soybean production,however,the traditional method was inefficient and time-consuming in selecting resistance cultivar.Molecular assistance breeding was an effective method for selecting resistance cultivar.GmRSCN3-2,a candidate gene,was cloned from the resistance cultivar'Dongnong L-10'according to the previous study,which was verified its function of candidate gene based on pCXSN-HA and Agrobacterium tumefaciens.The average number of females in the positive roots of the transgenic plants was significantly different from the average number of females in the blank control plants,which demonstrate the candidate gene was effective to control SCN Race 3.关键词
大豆/胞囊线虫病抗性/基因功能鉴定Key words
Soybean/Soybean cyst nematode resistance/Functional identification of gene分类
农业科技引用本文复制引用
谭云夫,宋伟,徐玲秀,孙秋霞,赵雪,韩英鹏..大豆胞囊线虫病3号生理小种的抗性候选基因GmRSCN3-2的初步鉴定[J].大豆科学,2017,36(6):862-865,4.基金项目
黑龙江省自然科学基金面上项目(C2015011) （C2015011）
国家自然科学基金(31671717) （31671717）
东北农业大学学术骨干计划项目(15XG04) （15XG04）
黑龙江省博士后项目(LBH-Z15017). （LBH-Z15017）
