南方医科大学学报2017，Vol.37Issue(9)：1156-1163,8.DOI:10.3969/j.issn.1673-4254.2017.09.03
高通量测序分析结肠腺瘤患者肠道黏膜菌群的构成变化
High-throughput sequencing for analysis of structural change of intestinal microbiota in patients with colorectal adenoma
摘要
Abstract
Objective To investigate the taxonomic richness and diversity of gut microbiota in patients with colorectal adenoma and elucidate the role of gut microorganisms in precancerous lesions in the colon and rectum.Method Adenomatous tissues from 31 patients with colorectal adenoma and normal intestinal mucosal tissues from 20 healthy control subjects were collected through colonoscopy.The total bacterial genomic DNA was extracted,and the V3-V4 hypervariable region in bacterial 16S rRNA gene was amplified using polymerase chain reaction and sequenced on an Illumina MiSeq platform.Results Patients with colorectal adenomas had a higher alpha diversity and richness indices compared to the healthy controls (P＜0.01).The mucosal microbiota in colorectal adenoma tissue showed a distinctive structural difference from that in normal intestinal mucosal tissues.At the phylum level,a large decrease in Firmicutes with concomitant relative expansion of Proteobacteria was observed in patients with colorectal adenomas,resulting in a significant decrease in the Firmicutes/Bacteroidetes ratio (P＜0.01).At the genus level,Lactococcus and Pseudomonas were enriched whereas Enterococcus,Bacillus,and Solibacillus were reduced obviously in the preneoplastic tissues (P＜0.01).We also found a similar gut microbiome composition between low-grade and high-grade intraepithelial neoplasia;the ratio of Escherichia-Shigella tended to increase in high-grade intraepithelial neoplasia,but this change was not statistically significant (P=0.28).Conclusion Significant changes in the structure of the intestinal flora occur in patients with colorectal adenomas,indicating that the association of dysbiosis of the gut microbiota with the occurrence of a pro-oncogenic microenvironment.关键词
结肠腺瘤/肠道菌群/结构失调/16SrRNA/高通量测序Key words
colorectal adenoma/gut microbiota/structural imbalance/16S rRNA/high-throughput引用本文复制引用
陆颖影,曾悦,胡国勇,王兴鹏..高通量测序分析结肠腺瘤患者肠道黏膜菌群的构成变化[J].南方医科大学学报,2017,37(9):1156-1163,8.基金项目
国家自然科学基金(81600500) （81600500）
上海交通大学医工交叉基金(YG2014ZD10)Supported by National Natural Science Foundation of China (81600500). （YG2014ZD10）
