南方医科大学学报2017，Vol.37Issue(9)：1178-1182,5.DOI:10.3969/j.issn.1673-4254.2017.09.06
邻苯二甲酸二-(2-乙基己)酯对血睾屏障完整性的损伤及机制
Effect of Di-(2-ethylhcxyl) phthalate exposure on blood-testis barrier integrity in rats
摘要
Abstract
Objective To investigate mechanism of di-(2-ethylhcxyl)phthalate (DEHP) exposure in causing blood-testis barrier (BTB) impairment in rats.Methods Two-months-old male SD rats were randomly divided into corn oil control group and DEHP (750 mg/kg) exposure group for daily intragastic treatment for 30 consecutive days.After the treatments the rats were examined for histomorphological changes of the testicle using HE staining and the expressions of the junction proteins N-cadherin β-catenin,ocdudin and connexin43 of the BTB using Western blot.In the in vitro study,the vitality and ROS generation level in Sertoli ceils exposed to different concentrations of DEHP were examined with MTT and ROS assay kits,respectively,and Nrf2 and p-p38 expressions were detected with Western blot.Results Compared with the control group,the rats with DEHP exposure showed structural damage of the seminiferous tubule and polarity loss of the spermatids.DEHP exposure caused significantly decreased expressions of occludin and connexin43 but increased expressions of N-cadherin and β-catenin in the testicle tissues of the rats (P＜0.05).The vitality of Sertoli cells was obviously decreased and ROS level increased significantly after exposure of the cells to increasing concentrations of DEHP,which also resulted in significantly up-regulated Nrf2 and p-p38 expressions (P＜0.05).Conclusions DEHP exposure causes increased oxidative stress in the Sertoli cells of the testis,activates p38 MAPK signaling pathway,and resuts eventually in impaired spermatogenesis in rats.关键词
血睾屏障/氧化应激损伤/p38/邻苯二甲酸二-(2-乙基己)酯Key words
di-(2-ethylhcxyl)phthalate/blood-testis barrier/spermatogenesis/oxidative stress/p38引用本文复制引用
沈炼桔,刘博,黄方圆,魏光辉,唐向亮,龙春兰,曹希宁,魏仪,王养才,孙茫,周玥,刘洋..邻苯二甲酸二-(2-乙基己)酯对血睾屏障完整性的损伤及机制[J].南方医科大学学报,2017,37(9):1178-1182,5.基金项目
国家自然科学基金(81370701) （81370701）
重庆市科委基础与前沿研究一般项目(cstc2017jcyjAX0438) （cstc2017jcyjAX0438）
重庆市教委科学技术研究项目(KJ1600229) （KJ1600229）
国家临床重点专科建设项目[国卫办医函(2013)554号]Supported by National Natural Science Foundation of China (81370701). （2013）
