南方医科大学学报2017，Vol.37Issue(9)：1183-1189,7.DOI:10.3969/j.issn.1673-4254.2017.09.07
siRNA干扰微管微丝交连因子1表达增强替莫唑胺诱导的胶质瘤细胞毒性
MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity
摘要
Abstract
Objective To investigate the role of microtubule-actin crosslinking factor 1 (MACF1) in the response of glioma cells to temozolomide (TMZ).Methods TMZ was applied to a human gliomablastoma cell line (U87) and changes in the protein expression and cellular localization were determined with Western blot,RT-PCR,and immunofluorescence.The responses of the cells with MACF1 expression knockdown by RNA interference to TMZ were assessed.TMZ-induced effects on MACF1 expression were also assessed by immunohistochemistry in a nude mouse model bearing human glioblastoma xenografts.Results TMZ resulted in significantly increased MACF1 expression (by about 2 folds) and changes in its localization in the gliomablastoma cells both in vitro and in vivo (P＜0.01).Knockdown of MACF1 reduced the proliferation (by 45％) of human glioma cell lines treated with TMZ (P＜0.01).TMZ-induced changes in MACF1 expression was accompanied by cytoskeletal rearrangement.Conclusion MACF1 may be a potential therapeutic target for glioblastoma.关键词
细胞骨架/微管微丝交连因子1/胶质母细胞瘤/替莫唑胺Key words
cytoskeleton rearrangement/microtubule-actin crosslinking factor 1/temozolomide/glioblastoma multiforme引用本文复制引用
谢思迪,陈子阳,王海,何敏毅,陆云涛,雷炳喜,李和珍,刘亚伟,漆松涛..siRNA干扰微管微丝交连因子1表达增强替莫唑胺诱导的胶质瘤细胞毒性[J].南方医科大学学报,2017,37(9):1183-1189,7.基金项目
国家自然基金(81372692,81302229,81472315) （81372692,81302229,81472315）
广东省自然科学基金(2014A030313167) （2014A030313167）
高校博士学科点专项科研基金(20134433120014) （20134433120014）
国家临床重点专科建设项目经费资助 （）
Supposed by National Natural Science Foundation of China (81372692,81302229,81472315). （81372692,81302229,81472315）
