南方医科大学学报2017，Vol.37Issue(9)：1190-1194,5.DOI:10.3969/j.issn.1673-4254.2017.09.08
脊髓5-羟色胺受体参与延髓外侧网状核对大鼠心脏伤害性感受的下行性抑制调控
Spinal serotonergic receptor is involved in descending inhibition of cardiac nociception by the lateral reticular nucleus in rats
摘要
Abstract
Objective To investigate the role of the lateral reticular nucleus (LRN) in descending inhibition of cardiac nociception in rats and the involvement of spinal serotonergic receptors in the descending inhibition.Methods Male SD rats were randomly divided into 5 groups,namely bradykinin (BK) group,BK + glutamate group,BK + methysergide group,BK + glutamate + methysergide group,and BK + glutamate + vehicle group.The rats received glutamate microinjection in the LRN combined with the intrathecal injection of serotonergic receptor antagonist methysergide,and the changes in the cardiacsomatic motor reflex induced by intrapericardial BK injection were monitored by observing electromyogram (EMG) responses of the dorsal spinotrapezius muscle;c-Fos expression in the spinal dorsal horn was also tested.Results Compared with BK group,intra-LRN glutamate administration produced a significant inhibitory effect on intrapericardial BK-induced EMG in a dose-dependent manner,and c-Fos expression was significantly decreased in the spinal dorsal horn (P＜0.05).Compared with BK + glutamate group,intrathecal administration of methysergide significantly attenuated the inhibitory effect of chemical stimulation of the LRN on intrapericardial BK-induced EMG and increased c-Fos expression in the spinal dorsal horn (P＜0.05).Intrathecal administration of the vehicle did not produce any effect on EMG or c-Fos expression (P＞0.05).Conclusion The serotonergic receptors in the spinal cord are involved in LRN-mediated descending inhibition of cardiac nociception in rats.关键词
5-羟色胺受体/延髓外侧网状核/心脏伤害性感受/下行性抑制Key words
serotonergic receptor/lateral reticular nucleus/cardic nociception/descending inhibition引用本文复制引用
韩曼,刘晓华,杜剑青..脊髓5-羟色胺受体参与延髓外侧网状核对大鼠心脏伤害性感受的下行性抑制调控[J].南方医科大学学报,2017,37(9):1190-1194,5.基金项目
国家自然科学基金(31171068) （31171068）
陕西省教育厅自然科学基金(320104-203010003,20140736) （320104-203010003,20140736）
陕西中医药大学博士基金(504010223)Supported by National Natural Science Foundation of China (31171068). （504010223）
