南方医科大学学报2017，Vol.37Issue(9)：1217-1221,5.DOI:10.3969/j.issn.1673-4254.2017.09.13
血管内皮生长因子与色素上皮衍生因子在糖尿病猕猴视网膜病变早期的表达
Expression of VEGF and PEDF in early-stage retinopathy in diabetic Macaca mulatta
摘要
Abstract
Objective To explore the expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in early stage of diabetic retinopathy (DR) in Macaca mulatta.Methods Three diabetic Macaca mulattas induced by high-fat diet were identified for early stage of diabetic retinopathy according to the fasting plasma glucose,hemoglobin Alc (HbA1c),fundus photograph and duration of diabetes,with another 3 age-matched healthy Macaca mulattas as control.The expression of VEGF and PEDF in the retinas of Macaca mulatta were detected by quantitative real-time PCR and immunohistochemistry.Result In early stage of diabetic retinopathy,VEGF mRNA and protein of the diabetic group were both significantly increased compared with the control group (P＜0.05).PEDF expression at both mRNA and protein levels was significantly decreased in diabetic Macaca mulattas compared with the control group (P＜0.01 and 0.05 respectively).Conclusion Retinal VEGF expression is increased and PEDF expression is decreased in early stage of diabetic retinopathy,suggesting their involvement in the occurrence of diabetic retinopathy and their value in assisting in the early diagnosis.关键词
糖尿病视网膜病变早期/猕猴/血管内皮生长因子/色素上皮衍生因子Key words
diabetic retinopathy/Macaca mulatta/vascular endothelial growth factor/pigment epithelium-derived factor引用本文复制引用
陈镜妃,罗启慧,黄超,刘文涛,曾文,高琪,陈苹,陈兵,陈正礼..血管内皮生长因子与色素上皮衍生因子在糖尿病猕猴视网膜病变早期的表达[J].南方医科大学学报,2017,37(9):1217-1221,5.基金项目
国家自然科学基金(31501200) （31501200）
国家重大科学仪器设备开发专项(2013YQ49085906) （2013YQ49085906）
国家科技支撑计划课题(2014BAI03801)Supported by National Natural Science Foundation of China (31501200). （2014BAI03801）
