南方医科大学学报2017，Vol.37Issue(9)：1228-1233,6.DOI:10.3969/j.issn.1673-4254.2017.09.15
丁苯酞对Aβ25-35诱导的阿尔茨海默病细胞模型的保护作用
Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells
摘要
Abstract
Objective To study the protective effect of butylphthalide in a cell model of Alzheimer's disease(AD) induced by Aβ 25-35 in Neuro 2a (N2a) cells.Methods N2a cells were divided into AD group,butylphthalide (NBP) group and control group.AD cell model was established by adding 20 μmol/L Aβ25-35 to cultured N2a cells.The cells in NBP group were treated with 0.1,1,10,or 100 μmol/L NBP 4 h prior to treatment with 20 μmol/L Aβ25-35.The cell viability were determined by MTr assay,the cell apoptotic rate were detected by AnnexinV-FITC flow cytometry,and the cell morphological changes were observed under inverted phase contrast microscope.The expression of TNF-α and IL-1β mRNA were determined by qRT-PCR.Results Compared with those in the control group,the number of adherent cells was significantly decreased,neurite structures were reduced,and the cell viability was decreased,while the apoptotic rate and expressions of TNF-α and IL-1β mRNA were increased in AD group (P＜0.05).Compared with that in AD group,the number of adherent cells was increased in NBP group and the cell morphology was similar to the normal control cells.The cell viability of N2a cells was increased in NBP group with decreased apoptotic rate and expression of TNF-αand IL-1β mRNA (P＜0.05).Conclusion Butylphthalide can protect against AD in the cell model induced by Aβ25-35 possibly by inhibiting the expression of inflammatory cytokines.关键词
丁苯酞/阿尔茨海默病/TNF-α/IL-1βKey words
butylphthalide/Alzheimer's disease/Aβ25-35/TNF-α/IL-1β引用本文复制引用
谢婧雯,赵艳,奉夏露,林芳波,罗静,刘鑫,侯德仁..丁苯酞对Aβ25-35诱导的阿尔茨海默病细胞模型的保护作用[J].南方医科大学学报,2017,37(9):1228-1233,6.基金项目
湖南省科技厅重点研发计划(2016JC2052) （2016JC2052）
中南大学湘雅三医院新湘雅人才工程“至善领跑计划”(20150305) （20150305）
