南方医科大学学报2017，Vol.37Issue(11)：1442-1447,6.DOI:10.3969/j.issn.1673-4254.2017.11.03
基于结核杆菌耐热抗原小分子多肽刺激人外周血T细胞产生TNF-α和IFN-γ鉴别肺结核和潜伏性结核感染
Peripheral blood T cell TNF-α and IFN-γ production stimulated by low molecular peptide of Mycobacterium tuberculosis heat-resistant antigen for differential diagnosis between pulmonary tuberculosis and latent tuberculosis infection
摘要
Abstract
Objective To investigate the effects of low molecular peptide of Mycobacterium tuberculosis heat-resistant antigen (Mtb-HAg-10k) on the production of tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) in peripheral blood T cells and test the feasibility of differential diagnosis between pulmonary tuberculosis (PTB) and latent tuberculosis infection (LTBI) by assessing the number of Mtb-HAg-10k-stimulated IFN-γ-producing T cells. Methods Peripheral blood mononuclear cells (PBMCs) were separated from the peripheral blood of 10 healthy adults, 6 individuals with LTBI and 13 patients with PTB. The PBMCs were cultured in the presence of Mtb-HAg-10k obtained by ultrafiltration centrifugation, with Mtb-HAg and phytohaemagglutinin (PHA) as the controls. The proportions of TNF-α-and IFN-γ-producing cells in the T cell subsets were detected by flow cytometry (FCM), and the number of IFN-γ-producing cells from patients with PTB and LTBI was detected with ELISPOT. Results Flow cytometry showed that Mtb-HAg-10k exposure resulted in a significantly higher proportion of TNF-α-producingγδT cells than that of IFN-γ-producingγδT cells in the PBMCs (P<0.01). Compared with the PBMCs exposed to PHA, the PBMCs exposed to Mtb-HAg-10k exhibited a significantly greater proportion of γδT cells that produced both TNF-α and IFN-γ (P<0.01) but a significantly lower proportion of αβT cells producing both TNF-α and IFN-γ (P<0.01). Mtb-HAg-10k exposure of the PBMCs caused a significant reduction in the number of IFN-γ-producing cells as compared with Mtb-HAg and PHA treatments (P<0.01), and this reduction was more obvious in PBMCs from patients with PTB than in those from individuals with LTBI (P<0.01). Conclusion Mtb-HAg-10k can markedly induce γδT cells in the PBMCs to produce TNF-α and IFN-γ, and detection of the number of IFN-γ-producing cells in the PBMCs following Mtb-HAg-10k stimulation helps in the differential diagnosis between pulmonary tuberculosis and latent tuberculosis infection.关键词
结核杆菌耐热抗原/γδT细胞/肿瘤坏死因子-α/干扰素-γ/肺结核/潜伏性结核感染Key words
Mycobacterium tuberculosis heat resistant antigen/γδT cells/tumor necrosis factor-α/interferon-γ/pulmonary tuberculosis/latent tuberculosis infection引用本文复制引用
唐洁,陈策,查成,常见荣,方强,王兆华,李柏青..基于结核杆菌耐热抗原小分子多肽刺激人外周血T细胞产生TNF-α和IFN-γ鉴别肺结核和潜伏性结核感染[J].南方医科大学学报,2017,37(11):1442-1447,6.基金项目
安徽高校科研平台创新团队项目(2016-40) （2016-40）
安徽高校省级科研项目(KJ2011Z248) （KJ2011Z248）
蚌埠医学院科技发展基金(BYKF1402) （BYKF1402）
