南方医科大学学报2017，Vol.37Issue(11)：1456-1460,5.DOI:10.3969/j.issn.1673-4254.2017.11.05
辛伐他汀通过上调动脉粥样硬化大鼠血管壁Bcl-2蛋白的表达而抑制细胞凋亡
Effects of simvastatin on aortic vascular endothelial cell apoptosis and Bcl-2 protein expression in a rat model of atherosclerosis
摘要
Abstract
Objective To explore the effects of simvastatin on vascular endothelial cell apoptosis and Bcl-2 protein expression in the aorta in a rat model of atherosclerosis. Methods Thirty-six rats were randomized into control group (n=10), atherosclerosis model group (n=13) and simvastatin intervention group (n=13). In the latter two groups, rat models of atherosclerosis were established by intraperitoneal injection of vitamin D3 combined with high-fat feeding for 6 weeks, and the control rats were fed with regular diet. In the intervention group, the rats were further fed with high-fat diet with daily simvastatin treatment for 4 weeks. After the treatments, the pathological changes and plaque in the thoracic aorta were observed, and the expression of Bcl-2 protein was detected with immunohistochemistry. TUNEL assay was used to determine the apoptosis index (AI) of the vascular endothelial cells. Results Compared with that in the control group, Bcl-2 protein expression in the aorta of atherosclerotic rats was significantly decreased (P<0.05); simvastatin treatment obviously increased the expression of Bcl-2 protein in atherosclerotic rats (P<0.05) to a level similar to that in the control group. The AI was the highest in the model group (P<0.05) and comparable between the control and simvastatin treatment group. Conclusion The therapeutic effect of simvastatin against atherosclerosis is probably mediated by up-regulation of Bcl-2 protein, which inhibits vascular endothelial cell apoptosis in rats with aortic atherosclerosis.关键词
动脉粥样硬化/细胞凋亡/辛伐他汀/Bcl-2/免疫组织化学Key words
atherosclerosis/apoptosis/simvastatin/Bcl-2/immunehistochemistry引用本文复制引用
胡司淦,李辉,康品方,陈天平,李妙男,朱建,高大胜,张恒,王洪巨..辛伐他汀通过上调动脉粥样硬化大鼠血管壁Bcl-2蛋白的表达而抑制细胞凋亡[J].南方医科大学学报,2017,37(11):1456-1460,5.基金项目
安徽高校研究重点项目(SK2016A0595) （SK2016A0595）
蚌埠市科技局课题(20150336) （20150336）
蚌埠医学院科研课题资助(BY0855) （BY0855）
