南方医科大学学报2017，Vol.37Issue(11)：1489-1493,5.DOI:10.3969/j.issn.1673-4254.2017.11.10
醛固酮对成骨细胞增殖分化及成骨相关基因表达的影响
Effects of aldosterone on osteoblast proliferation, differentiation and osteogenic gene expressions in vitro
摘要
Abstract
Objective To study the effect of aldosterone on cell proliferation, alkaline phosphatase (AKP) activity and osteogenic gene expression in rat osteoblasts and explore the mechanisms. Methods Osteoblasts isolated from the skull of neonatal SD rats by enzyme digestion were cultured and treated with different concentrations of aldosterone. The cell proliferation and AKP activity were evaluated using CCK-8 assay kit and AKP assay kit, respectively. The effects of aldosterone on mRNA and protein expressions of the osteogenic genes and epithelial sodium channel (ENaC) gene were investigated using semi-quantitative PCR and Western blotting. Results Compared with the control cells, the cells treated with 0.01-1.0 μmol/L aldosterone showed obviously enhanced proliferation while lower (1 × 10-3 μmol/L) or higher (10 μmol/L) concentrations of aldosterone did not significantly affect the cell proliferation. Aldosterone within the concentration range of 1× 10-3 to 10 μmol/L did not cause significant changes in AKP activity in the osteoblasts. Treatment with 0.01 to 1.0 μmol/L aldosterone significantly upregulated the expressions of the osteogenic genes andα-ENaC gene at both the mRNA and protein levels. Conclusion Aldosterone within the concentration range of 0.01-1.0 μmol/L stimulates the proliferation and osteogenic gene expressions and enhances α-ENaC gene expression in rat osteoblasts in vitro, suggesting the possibility that ENaC participates in aldosterone-mediated regulation of osteoblast functions.关键词
醛固酮/成骨细胞/成骨功能/上皮钠离子通道Key words
aldosterone/osteoblasts/osteogenic function/epithelial sodium channel引用本文复制引用
陈珺,谢芳梅,林鑫,林思慧,杨国柱,卢丽,陆幸妍,李青南..醛固酮对成骨细胞增殖分化及成骨相关基因表达的影响[J].南方医科大学学报,2017,37(11):1489-1493,5.基金项目
国家自然科学基金青年基金项目(81400853) （81400853）
广东省普通高校国际暨港澳台合作创新平台及国际合作重大项目(项目编号2015KGJHZ022) （项目编号2015KGJHZ022）
中央财政支持地方高校发展专项资金(粤财政[2016] 202号) Supported by National Natural Science Foundation of China (81400853). （粤财政[2016] 202号）
