南方医科大学学报2017，Vol.37Issue(11)：1506-1511,6.DOI:10.3969/j.issn.1673-4254.2017.11.13
右美托咪定后处理对糖尿病大鼠心肌缺血再灌注损伤及炎症反应的影响
Effect of dexmedetomidine postconditioning on myocardial ischemia-reperfusion injury and inflammatory response in diabetic rats
摘要
Abstract
Objective To investigate the effect of dexmedetomidine postconditioning in alleviating myocardial ischemia-reperfusion (IR) injury and inflammation in diabetic mellitus rats. Methods Thirty normal male Sprauge Dawley (SD) rats were randomly allocated into 3 groups (n=10), namely the sham-operated group, IR group, and dexmedetomidine postconditioning (DP) group. Similarly, another thirty diabetic SD rats were also randomly allocated into diabetic sham (DM-S) group, diabetic IR (DM-IR) group and diabetic dexmedetomidine postconditioning (DM-DP) group. The mean arterial pressure (MAP), heart rate (HR) and the rate pressure product (RPP) were recorded at baseline, after 30 min of ischemia, and at 30 and 120 min during reperfusion. Myocardial infarct size was analyzed by TTC double staining method, and plasma levels of CTnI, TNF-a, IL-6, IL-10 and IL-1β were measured at 120 min of reperfusion. Results Compared with those in the sham-operated group, normal and diabetic rats in IR and DP groups showed significantly lowered MAP, HR, and RPP and increased levels of plasma CTnI, TNF-a, IL-6, IL-10 and IL-1β levels after 30 min of ischemia and at 30 min and 120 min of reperfusion (P<0.05). Compared with those in the IR group, the normal rats in DP group showed decreased MAP, HR, and RPP at 30 min of ischemia and at 30 min of reperfusion, which increased at 120 min of reperfusion (P<0.05); the infarct size and plasma CTnI, TNF-a, IL-6 and IL-1β levels were decreased while IL-10 was increased in DP group (P<0.05). Compared with those in DP group, the rats in DM-DP group showed similar MAP, HR and RPP (P>0.05) but significantly increased infarct size and plasma CTnI, TNF-a, IL-6 and IL-1β levels (P<0.05).Conclusion Dexmedetomidine postconditioning may produce a cardioprotective effect against myocardial IR injury in normal rats by alleviating inflammation, but can not reduce the release of inflammatory mediators in diabetic rats to improve myocardial infarction.关键词
心肌缺血再灌注损伤/右美托咪定/2型糖尿病/炎症Key words
myocardial reperfusion injury/dexmedetomidine/diabetes mellitus/type2/inflammation引用本文复制引用
胡静,谷小雨,孟炎,王娅,高琴,李正红,李晓红,程向阳..右美托咪定后处理对糖尿病大鼠心肌缺血再灌注损伤及炎症反应的影响[J].南方医科大学学报,2017,37(11):1506-1511,6.基金项目
安徽省自然科学基金(1508085MH169,1508085MH170) （1508085MH169,1508085MH170）
蚌埠医学院研究生科研创新计划项目(Byycx1625) （Byycx1625）
