南方医科大学学报2017，Vol.37Issue(11)：1512-1516,5.DOI:10.3969/j.issn.1673-4254.2017.11.14
Survivin和PI3K、AKT在寻常型银屑病皮损角质形成细胞中的表达及其相关性
Expressions of survivin, PI3K and AKT in keratinocytes in skin lesions and their pathogenic role in psoriasis vulgaris
摘要
Abstract
Objective To explore the role of survivin and PI3K/AKT pathway in the pathogenesis of psoriasis vulgaris (PV). Methods Plaque-like lesions collected from 22 patients with PV in progressive stage and 18 normal control skin specimens were examined using immunohistochemical staining, Western blotting and real-time quantitative PCR for expressions of survivin, PI3K and AKT in the keratinocytes, and their correlation was analyzed. A small interfering RNA (siRNA) was used to knock down AKT in cultured HaCaT cells, and Western blotting was used to detect the changes in the expression of survivin. Results Compared with normal skin, PV lesions showed obviously up-regulated expressions of survivin, PI3K and AKT in the keratinocytes. Survivin expression was positively correlated with PI3K (r=0.4510, P=0.0351) and AKT (r=0.4423, P=0.0393) in the keratinocytes in PV lesions. In cultured HaCaT cells, siRNA-mediated knockdown of AKT caused down-regulation of survivin expression. Conclusion Survivin and PI3K/AKT signaling pathway may participate in the occurrence and progression of PV.关键词
Survivin/PI3K/AKT/寻常型银屑病Key words
Survivin/PI3K/AKT/psoriasis vulgaris引用本文复制引用
王昊,冉立伟,惠珂,王新阳,郑焱..Survivin和PI3K、AKT在寻常型银屑病皮损角质形成细胞中的表达及其相关性[J].南方医科大学学报,2017,37(11):1512-1516,5.基金项目
国家自然科学基金(30960349) Supported by National Natural Science Foundation of China (30960349). （30960349）
